Natural spring water gargle samples as an alternative to nasopharyngeal swabs for SARS-CoV-2 detection using a laboratory-developed test.
COVID-19
PCR
SARS-CoV-2
diagnosis
gargle
Journal
Journal of medical virology
ISSN: 1096-9071
Titre abrégé: J Med Virol
Pays: United States
ID NLM: 7705876
Informations de publication
Date de publication:
03 2022
03 2022
Historique:
revised:
28
09
2021
received:
18
08
2021
accepted:
18
10
2021
pubmed:
22
10
2021
medline:
7
6
2022
entrez:
21
10
2021
Statut:
ppublish
Résumé
The objective of this study was to validate the use of spring water gargle (SWG) as an alternative to oral and nasopharyngeal swab (ONPS) for SARS-CoV-2 detection with a laboratory-developed test. Healthcare workers and adults from the general population, presenting to one of two COVID-19 screening clinics in Montréal and Québec City, were prospectively recruited to provide a gargle sample in addition to the standard ONPS. The paired specimens were analyzed using thermal lysis followed by a laboratory-developed nucleic acid amplification test (LD-NAAT) to detect SARS-CoV-2, and comparative performance analysis was performed. An individual was considered infected if a positive result was obtained on either sample. A total of 1297 adult participants were recruited. Invalid results (n = 18) were excluded from the analysis. SARS-CoV-2 was detected in 144/1279 (11.3%) participants: 126 from both samples, 15 only from ONPS, and 3 only from SWG. Overall, the sensitivity was 97.9% (95% CI: 93.7-99.3) for ONPS and 89.6% (95% CI: 83.4-93.6; p = 0.005) for SWG. The mean ONPS cycle threshold (C
Identifiants
pubmed: 34672374
doi: 10.1002/jmv.27407
pmc: PMC8661969
doi:
Substances chimiques
Mouthwashes
0
Water
059QF0KO0R
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
985-993Informations de copyright
© 2021 Wiley Periodicals LLC.
Références
Infect Control Hosp Epidemiol. 2021 Feb;42(2):248-249
pubmed: 32389132
Euro Surveill. 2020 Jan;25(3):
pubmed: 31992387
Clin Infect Dis. 2020 Dec 17;71(10):2663-2666
pubmed: 32442256
J Med Virol. 2022 Mar;94(3):985-993
pubmed: 34672374
Front Med (Lausanne). 2020 Aug 04;7:465
pubmed: 32903849
Infect Control Hosp Epidemiol. 2021 Nov;42(11):1340-1344
pubmed: 33436122
N Engl J Med. 2020 May 28;382(22):2081-2090
pubmed: 32329971
Indian J Med Res. 2020 Jul & Aug;152(1 & 2):77-81
pubmed: 32820725
J Med Virol. 2021 Dec;93(12):6837-6840
pubmed: 34324212
J Virol Methods. 2021 Sep;295:114184
pubmed: 34029634
Clin Infect Dis. 2021 Mar 15;72(6):1064-1066
pubmed: 32584972
N Engl J Med. 2020 May 28;382(22):e76
pubmed: 32302471
Virol J. 2020 Dec 18;17(1):196
pubmed: 33339539
Microbiol Spectr. 2021 Sep 3;9(1):e0036121
pubmed: 34259547
Emerg Infect Dis. 2020 Oct;26(10):2497-2499
pubmed: 32497001
Nat Rev Microbiol. 2021 Mar;19(3):171-183
pubmed: 33057203
Clin Infect Dis. 2020 Jul 28;71(15):893-894
pubmed: 32241023
Clin Infect Dis. 2021 Apr 8;72(7):1253-1255
pubmed: 32596725
Nature. 2020 May;581(7809):465-469
pubmed: 32235945
Front Med (Lausanne). 2020 Sep 18;7:549860
pubmed: 33043036
PLoS One. 2021 Feb 26;16(2):e0247792
pubmed: 33635936
Infection. 2021 Jun;49(3):527-531
pubmed: 33738680
J Virol Methods. 2021 Jul;293:114131
pubmed: 33798606
J Clin Virol. 2021 Nov;144:104995
pubmed: 34619381
J Clin Virol. 2020 Nov;132:104615
pubmed: 32927356
J Med Virol. 2021 Sep;93(9):5333-5338
pubmed: 33851739
Oral Dis. 2021 Jun 3;:
pubmed: 34080272
Clin Infect Dis. 2020 Jun 16;:
pubmed: 32556191
Biometrics. 1977 Mar;33(1):159-74
pubmed: 843571
J Clin Microbiol. 2021 Mar 19;59(4):
pubmed: 33514627
Pathol Res Pract. 2021 Apr;220:153381
pubmed: 33640711