CRISPR-Cas9 mediated knockout of AnxA6 gene enhances influenza A virus replication in low-permissive HEK293FT cell line.
Annexin A6
CRISPR-Cas9-mediated knockout
Genome editing
Influenza virus
Permissive cell line
Journal
Gene
ISSN: 1879-0038
Titre abrégé: Gene
Pays: Netherlands
ID NLM: 7706761
Informations de publication
Date de publication:
30 Jan 2022
30 Jan 2022
Historique:
received:
02
04
2021
revised:
26
07
2021
accepted:
14
10
2021
pubmed:
22
10
2021
medline:
15
12
2021
entrez:
21
10
2021
Statut:
ppublish
Résumé
Using cell cultures of human origin for the propagation of influenza virus is an attractive way to preserve its glycosylation profile and antigenic properties, which is essential in influenza surveillance and vaccine production. However, only few cell lines are highly permissive to influenza virus, and none of them are of human origin. The barrier might be associated with host restriction factors inhibiting influenza growth, such as AnxA6 protein counteracting the process of influenza virion packaging. In the presented work we explore the CRISPR-Cas9 mediated knockout of ANXA6 gene as a way to overcome the host restriction barrier and increase the susceptibility of human cell line to influenza infection. By CRISPR-Cas9 genome editing we modified HEK293FT cells and obtained several clones defective in the ANXA6 gene. The replication of the influenza A virus in original HEK293FT cells and the HEK293FT-ANXA6
Identifiants
pubmed: 34673207
pii: S0378-1119(21)00619-3
doi: 10.1016/j.gene.2021.146024
pii:
doi:
Substances chimiques
Annexin A6
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
146024Informations de copyright
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