StemBond hydrogels control the mechanical microenvironment for pluripotent stem cells.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
21 10 2021
21 10 2021
Historique:
received:
04
10
2019
accepted:
22
09
2021
entrez:
22
10
2021
pubmed:
23
10
2021
medline:
15
12
2021
Statut:
epublish
Résumé
Studies of mechanical signalling are typically performed by comparing cells cultured on soft and stiff hydrogel-based substrates. However, it is challenging to independently and robustly control both substrate stiffness and extracellular matrix tethering to substrates, making matrix tethering a potentially confounding variable in mechanical signalling investigations. Moreover, unstable matrix tethering can lead to poor cell attachment and weak engagement of cell adhesions. To address this, we developed StemBond hydrogels, a hydrogel in which matrix tethering is robust and can be varied independently of stiffness. We validate StemBond hydrogels by showing that they provide an optimal system for culturing mouse and human pluripotent stem cells. We further show how soft StemBond hydrogels modulate stem cell function, partly through stiffness-sensitive ERK signalling. Our findings underline how substrate mechanics impact mechanosensitive signalling pathways regulating self-renewal and differentiation, indicating that optimising the complete mechanical microenvironment will offer greater control over stem cell fate specification.
Identifiants
pubmed: 34675200
doi: 10.1038/s41467-021-26236-5
pii: 10.1038/s41467-021-26236-5
pmc: PMC8531294
doi:
Substances chimiques
Hydrogels
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6132Subventions
Organisme : Medical Research Council
ID : MR/M011089/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/R017735/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 203151/Z/16/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : WT101861
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/K017047/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : G1100312/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/R015635/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : G1100312
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_PC_17230
Pays : United Kingdom
Informations de copyright
© 2021. The Author(s).
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