Enhanced ATP and antioxidant levels for cAMP biosynthesis by Arthrobacter sp. CCTCC 2013431 with polyphosphate addition.
Cyclic adenosine monophosphate
Energy metabolism
Oxidative stress
Polyphosphate
Salvage synthesis
Journal
Biotechnology letters
ISSN: 1573-6776
Titre abrégé: Biotechnol Lett
Pays: Netherlands
ID NLM: 8008051
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
received:
02
09
2021
accepted:
13
10
2021
pubmed:
23
10
2021
medline:
1
3
2022
entrez:
22
10
2021
Statut:
ppublish
Résumé
When citrate and pyruvate were utilized to strengthen ATP generation for high cAMP production, oxidative stress became more severe in cells resulting in lower cell viability and cAMP formation at the late fermentation phase. To further improve cAMP biosynthesis, the effects of polyphosphate on cAMP fermentation performance together with intracellular ATP and oxidation levels were investigated under high oxidative stress condition and then high efficient cAMP fermentation process based on polyphosphate and salvage synthesis was developed and studied. With 2 g/L-broth sodium hexametaphosphate added at 24 h was determined as the optimal condition for cAMP production by Arthrobacter sp. CCTCC 2013431 in shake flasks. Under high oxidative stress condition caused by adding 15 mg/L-broth menadione, cAMP contents and cell viability were improved greatly due to hexametaphosphate addition and also exceeded those of control (without hexametaphosphate and menadione added) when fermentations were conducted in a 7 L bioreactor. Meanwhile, ATP levels and antioxidant capacity were improved obviously by hexametaphosphate as well. Moreover, a fermentation process with hexametaphosphate and hypoxanthine coupling added was developed by which cAMP concentration reached 7.25 g/L with an increment of 87.1% when compared with only hypoxanthine added batch and the high ROS contents generated from salvage synthesis were reduced significantly. Polyphosphate could improve intracellular ATP levels and antioxidant capacity significantly under high oxidative stress condition resulting in enhanced cell viability and cAMP fermentation production no matter by de novo synthesis or salvage synthesis.
Identifiants
pubmed: 34676500
doi: 10.1007/s10529-021-03197-9
pii: 10.1007/s10529-021-03197-9
doi:
Substances chimiques
Antioxidants
0
Phosphates
0
Polyphosphates
0
Adenosine Triphosphate
8L70Q75FXE
Cyclic AMP
E0399OZS9N
sodium polymetaphosphate
P1BM4ZH95L
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2223-2231Subventions
Organisme : Doctoral Scientific Research Foundation of Henan institute of science and technology
ID : 2015006
Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer Nature B.V.
Références
Emanuel SL, Devaki B, Arthur RG et al (2020) Polyphosphate: a multifunctional metabolite in cyanobacteria and algae. Front Plant Sci 11:938. https://doi.org/10.3389/fpls.2020.00938
doi: 10.3389/fpls.2020.00938
Li S, Ji J, Hu S et al (2020) Enhancement of ε-poly-L-lysine production in Streptomyces griseofuscus by addition of exogenous astaxanthin. Bioproc Biosyst Eng 43(10):1813–1821
doi: 10.1007/s00449-020-02372-y
Li Z, Chen B, Fang Z et al (2018) A novel fermentation process for cyclic adenosine monophosphate production based on citrate coupling hypoxanthine addition in pulses. Food Ferment Ind 44(11):154–158
Li Z, Chen B, Gu Y et al (2021a) Enhanced endogenous amino acids and energy metabolism level for cAMP biosynthesis by Arthrobacter sp. CCTCC 2013431 with citrate as cosubstrate. Biotechnol Lett. https://doi.org/10.1007/s10529-021-03170-6
doi: 10.1007/s10529-021-03170-6
pubmed: 34698972
pmcid: 8510960
Li Z, Zhang C, Tan H et al (2021b) Enhancing cAMP fermentation formation via glutathione and auxiliary energy substance coupling addition. Sci Technol Food Ind. https://doi.org/10.13386/j.issn1002-0306.2021010246
doi: 10.13386/j.issn1002-0306.2021010246
Liu Q, Wu C, Huang S et al (2018) Decreased hyperpolarization-activated cyclic nucleotide-gated channels are involved in bladder dysfunction associated with spinal cord injury. Int J Mol Med 41(5):2609–2618
pubmed: 29436607
pmcid: 5846662
Maureen CT, Marisol Z, Anton G et al (2019) Malaria inflammation by xanthine oxidase-produced reactive oxygen species. EMBO Mol Med 11:e9903. https://doi.org/10.15252/emmm.201809903
doi: 10.15252/emmm.201809903
Niu HQ, Wang JZ, Zhuang W et al (2018) Comparative transcriptomic and proteomic analysis of Arthrobacter sp CGMCC 3584 responding to dissolved oxygen for cAMP production. Sci Rep 8(1):1–13
Niu HQ, Sun XZ, Song JR et al (2020) Knockout of pde gene in Arthrobacter sp. CGMCC 3584 and transcriptomic analysis of its effects on cAMP production. Bioproc Biosyst Eng 43(5):839–850
doi: 10.1007/s00449-019-02280-w
Resnick SM, Zehnder AJ (2000) In vitro ATP regeneration from polyphosphate and AMP by polyphosphate:AMP phosphotransferase and adenylate kinase from Acinetobacter johnsonii 210A. Appl Environ Microb 66(5):2045–2051
doi: 10.1128/AEM.66.5.2045-2051.2000
Shao HH, Tu YY, Wang YJ et al (2019) Oxidative stress response of Aspergillus oryzae induced by hydrogen peroxide and menadione sodium bisulfite. Microorganisms 7(8):225–237
doi: 10.3390/microorganisms7080225
Tabassum N, Kheya IS, Ibn SA et al (2020) A review on the possible leakage of electrons through the electron transport chain within mitochondria. J Biomed Res Environ Sci 6:105–113
doi: 10.37871/jels1127
Tang-Fichaux M, Chagneau CV, Bossuet-Greif N et al (2020) The polyphosphate kinase of Escherichia coli is required for full production of the genotoxin colibactin. mSphere 5:e01195-e1220. https://doi.org/10.1128/mSphere.01195-20
doi: 10.1128/mSphere.01195-20
pubmed: 33328353
pmcid: 7771237
Xiao Q, Niu JR, Liu H et al (2019) High conversion of D-fructose into D-allulose by enzymes coupling with an ATP regeneration system. Mol Biotechnol 61:432–441
doi: 10.1007/s12033-019-00174-6
Yan P, Sun HB, Lu PQ et al (2018) Enhancement of ε-poly-L-lysine synthesis in Streptomyces by exogenous glutathione. Bioprocess Biosyst Eng 41:129–134
doi: 10.1007/s00449-017-1849-0
Yu L, Wu J, Liu J et al (2011) Enhanced curdlan production in Agrobacterium sp. ATCC 31749 by addition of low-polyphosphates. Biotechnol Bioproc E 16:34–41
doi: 10.1007/s12257-010-0145-5
Zeng X, Chen XS, Gao Y et al (2015) Continuously high reactive oxygen species generation decreased the specific ε-poly-L-lysine formation rate in fed-batch fermentation using glucose and glycerol as a mixed carbon source. Process Biochem 50(12):1993–2003
doi: 10.1016/j.procbio.2015.09.012