Simultaneous detection and differentiation between Mycobacterium leprae and Mycobacterium lepromatosis using novel polymerase chain reaction primers.
Mycobacterium leprae
Mycobacterium lepromatosis
rpoT
Co-infection
Molecular diagnostic test
Polymerase chain reaction
Journal
The Journal of dermatology
ISSN: 1346-8138
Titre abrégé: J Dermatol
Pays: England
ID NLM: 7600545
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
revised:
20
08
2021
received:
09
07
2021
accepted:
07
09
2021
pubmed:
23
10
2021
medline:
15
12
2021
entrez:
22
10
2021
Statut:
ppublish
Résumé
We have developed a polymerase chain reaction-based method to detect and distinguish Mycobacterium leprae and Mycobacterium lepromatosis using a single set of primers based on a 45-bp difference in the amplicon size of their rpoT gene. This method can also help in detecting the cases of co-infection in a single experiment.
Identifiants
pubmed: 34676580
doi: 10.1111/1346-8138.16165
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1936-1939Subventions
Organisme : Leprosy Research Initiative, Netherland
Organisme : R2STOP (effect:hope TLM) Canada
Informations de copyright
© 2021 Japanese Dermatological Association.
Références
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