The Validation of a Non-Invasive Skin Sampling Device for Detecting Cetacean Poxvirus.
DNA extraction
PCR
biopsy
cetacean poxvirus
cetaceans
cytology cell sampler
health indicator
skin lesions
welfare
Journal
Animals : an open access journal from MDPI
ISSN: 2076-2615
Titre abrégé: Animals (Basel)
Pays: Switzerland
ID NLM: 101635614
Informations de publication
Date de publication:
27 Sep 2021
27 Sep 2021
Historique:
received:
28
07
2021
revised:
16
09
2021
accepted:
24
09
2021
entrez:
23
10
2021
pubmed:
24
10
2021
medline:
24
10
2021
Statut:
epublish
Résumé
Poxvirus-like lesions are widely used as a potential health indicator in cetaceans, although for this application, corroboration of Poxvirus skin disease is imperative. Aiming to address skin biopsies intrusiveness, a preliminary investigation of a non-invasive skin sampling procedure to molecularly detect CePV-1 in 12 tattoo-like-lesions from two free-ranging stranded cetaceans in the Canary Islands was performed. Skin lesions were brushed with cytology cell samplers (CCSs) and placed into 1.5 mL microcentrifuge tubes with 1 mL of RNA
Identifiants
pubmed: 34679836
pii: ani11102814
doi: 10.3390/ani11102814
pmc: PMC8532937
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : National Project of Canary Islands Government
ID : CGL2015-71498-P, PGC2018-101226-B-I00, MAC/1.1b/149
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