Identification of Binding Proteins for TSC22D1 Family Proteins Using Mass Spectrometry.
Alternative Splicing
Cell Differentiation
Cell Line
Cell Nucleus
/ metabolism
Cytoplasm
/ metabolism
DNA Damage
GTP-Binding Proteins
/ metabolism
HEK293 Cells
Histones
/ metabolism
Humans
Mass Spectrometry
Mitochondria
/ metabolism
Nuclear Proteins
/ metabolism
Protein Binding
Protein Interaction Maps
Repressor Proteins
/ metabolism
Guanine nucleotide-binding protein-like 3 (GNL3)
Histone H1
TSC22D1 family proteins
binding protein
differentiation-inducing therapy
mass spectrometry
molecular-targeted therapy
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
09 Oct 2021
09 Oct 2021
Historique:
received:
08
09
2021
revised:
30
09
2021
accepted:
06
10
2021
entrez:
23
10
2021
pubmed:
24
10
2021
medline:
15
12
2021
Statut:
epublish
Résumé
TSC-22 (TGF-β stimulated clone-22) has been reported to induce differentiation, growth inhibition, and apoptosis in various cells. TSC-22 is a member of a family in which many proteins are produced from four different family genes. TSC-22 (corresponding to TSC22D1-2) is composed of 144 amino acids translated from a short variant mRNA of the TSC22D1 gene. In this study, we attempted to determine the intracellular localizations of the TSC22D1 family proteins (TSC22D1-1, TSC-22 (TSC22D1-2), and TSC22(86) (TSC22D1-3)) and identify the binding proteins for TSC22D1 family proteins by mass spectrometry. We determined that TSC22D1-1 was mostly localized in the nucleus, TSC-22 (TSC22D1-2) was localized in the cytoplasm, mainly in the mitochondria and translocated from the cytoplasm to the nucleus after DNA damage, and TSC22(86) (TSC22D1-3) was localized in both the cytoplasm and nucleus. We identified multiple candidates of binding proteins for TSC22D1 family proteins in in vitro pull-down assays and in vivo binding assays. Histone H1 bound to TSC-22 (TSC22D1-2) or TSC22(86) (TSC22D1-3) in the nucleus. Guanine nucleotide-binding protein-like 3 (GNL3), which is also known as nucleostemin, bound to TSC-22 (TSC22D1-2) in the nucleus. Further investigation of the interaction of the candidate binding proteins with TSC22D1 family proteins would clarify the biological roles of TSC22D1 family proteins in several cell systems.
Identifiants
pubmed: 34681573
pii: ijms222010913
doi: 10.3390/ijms222010913
pmc: PMC8536140
pii:
doi:
Substances chimiques
GNL3 protein, human
0
Histones
0
Nuclear Proteins
0
Repressor Proteins
0
TSC22D1 protein, human
0
GTP-Binding Proteins
EC 3.6.1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Dokkyo Medical University
ID : No.2019-13
Organisme : Dokkyo Medical University
ID : No.2020-18
Organisme : Dokkyo Medical University
ID : 00021102
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