Characterization of Fungal FAD-Dependent AA3_2 Glucose Oxidoreductases from Hitherto Unexplored Phylogenetic Clades.
CAZy AA3_2
GMC oxidoreductase
glucose dehydrogenase
glucose oxidase
substrate specificity
Journal
Journal of fungi (Basel, Switzerland)
ISSN: 2309-608X
Titre abrégé: J Fungi (Basel)
Pays: Switzerland
ID NLM: 101671827
Informations de publication
Date de publication:
17 Oct 2021
17 Oct 2021
Historique:
received:
30
08
2021
revised:
09
10
2021
accepted:
11
10
2021
entrez:
23
10
2021
pubmed:
24
10
2021
medline:
24
10
2021
Statut:
epublish
Résumé
The CAZy auxiliary activity family 3 (AA3) comprises FAD-dependent enzymes belonging to the superfamily of glucose-methanol-choline (GMC) oxidoreductases. Glucose oxidase (GOx; EC 1.1.3.4) and glucose dehydrogenase (GDH; EC 1.1.5.9) are part of subfamily AA3_2 and catalyze the oxidation of β-D-glucose at its anomeric carbon to D-glucono-1,5-lactone. Recent phylogenetic analysis showed that AA3_2 glucose oxidoreductases can be grouped into four major clades, GOx I and GDH I-III, and in minor clades such as GOx II or distinct subclades. This wide sequence space of AA3_2 glucose oxidoreductases has, however, not been studied in detail, with mainly members of GOx I and GDH I studied biochemically or structurally. Here, we report the biochemical characterization of four fungal glucose oxidoreductases from distinct, hitherto unexplored clades or subclades. The enzyme from
Identifiants
pubmed: 34682294
pii: jof7100873
doi: 10.3390/jof7100873
pmc: PMC8537048
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Austrian Science Fund FWF
ID : W 1224
Pays : Austria
Organisme : Austrian Science Fund
ID : P 33545
Organisme : Indonesia-Austria Scholarship Programme (IASP), a joint scholarship between the Indonesian Ministry of Education and Culture (KEMDIKBUD) and Austria's Agency for Education and In-ternationalization (OeAD-GmbH) in corporation with ASEAN European Academic U
ID : 0
Organisme : Austrian Science Fund
ID : Doctoral Programme BioToP-Biomolecular Technology of Proteins (FWF W1224)
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