Enhancing Antibodies' Binding Capacity through Oriented Functionalization of Plasmonic Surfaces.
IgG
biosensor
metal enhanced fluorescence
plasmonic surface
surface functionalization
Journal
Nanomaterials (Basel, Switzerland)
ISSN: 2079-4991
Titre abrégé: Nanomaterials (Basel)
Pays: Switzerland
ID NLM: 101610216
Informations de publication
Date de publication:
05 Oct 2021
05 Oct 2021
Historique:
received:
09
08
2021
revised:
27
09
2021
accepted:
30
09
2021
entrez:
23
10
2021
pubmed:
24
10
2021
medline:
24
10
2021
Statut:
epublish
Résumé
Protein A has long been used in different research fields due to its ability to specifically recognize immunoglobulins (Ig). The protein derived from Staphylococcus aureus binds Ig through the Fc region of the antibody, showing its strongest binding in immunoglobulin G (IgG), making it the most used protein in its purification and detection. The research presented here integrates, for the first time, protein A to a silicon surface patterned with gold nanoparticles for the oriented binding of IgG. The signal detection is conveyed through a metal enhanced fluorescence (MEF) system. Orienting immunoglobulins allows the exposition of the fragment antigen-binding (Fab) region for the binding to its antigen, substantially increasing the binding capacity per antibody immobilized. Antibodies orientation is of crucial importance in many diagnostics devices, particularly when either component is in limited quantities.
Identifiants
pubmed: 34685056
pii: nano11102620
doi: 10.3390/nano11102620
pmc: PMC8538552
pii:
doi:
Types de publication
Journal Article
Langues
eng
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