Protein Hydration in a Bioprotecting Mixture.
hydration water
light scattering
lysozyme
trehalose
Journal
Life (Basel, Switzerland)
ISSN: 2075-1729
Titre abrégé: Life (Basel)
Pays: Switzerland
ID NLM: 101580444
Informations de publication
Date de publication:
22 Sep 2021
22 Sep 2021
Historique:
received:
31
07
2021
revised:
10
09
2021
accepted:
18
09
2021
entrez:
23
10
2021
pubmed:
24
10
2021
medline:
24
10
2021
Statut:
epublish
Résumé
We combined broad-band depolarized light scattering and infrared spectroscopies to study the properties of hydration water in a lysozyme-trehalose aqueous solution, where trehalose is present above the concentration threshold (30% in weight) relevant for biopreservation. The joint use of the two different techniques, which were sensitive to inter-and intra-molecular degrees of freedom, shed new light on the molecular mechanism underlying the interaction between the three species in the mixture. Thanks to the comparison with the binary solution cases, we were able to show that, under the investigated conditions, the protein, through preferential hydration, remains strongly hydrated even in the ternary mixture. This supported the water entrapment scenario, for which a certain amount of water between protein and sugar protects the biomolecule from damage caused by external agents.
Identifiants
pubmed: 34685367
pii: life11100995
doi: 10.3390/life11100995
pmc: PMC8537178
pii:
doi:
Types de publication
Journal Article
Langues
eng
Références
J Phys Chem B. 2008 Nov 13;112(45):14273-80
pubmed: 18942780
Int J Biol Macromol. 2012 Jan 1;50(1):230-5
pubmed: 22085755
Soft Matter. 2016 Jul 7;12(25):5501-14
pubmed: 27280176
Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip Topics. 1999 Nov;60(5 Pt A):5505-21
pubmed: 11970425
Biophys J. 2003 Sep;85(3):1903-13
pubmed: 12944303
Int J Mol Sci. 2021 Mar 02;22(5):
pubmed: 33801421
Biopolymers. 1994 Jul;34(7):957-61
pubmed: 8054473
Phys Chem Chem Phys. 2021 Jul 21;23(28):15020-15029
pubmed: 34190269
J Phys Chem Lett. 2013 Apr 4;4(7):1188-92
pubmed: 26282040
J Chem Phys. 2011 Feb 7;134(5):055104
pubmed: 21303164
J Am Chem Soc. 2019 Jul 10;141(27):10569-10580
pubmed: 31117647
Biophys Chem. 1997 Feb 28;64(1-3):25-43
pubmed: 9127936
J Phys Chem Lett. 2014 Apr 3;5(7):1181-6
pubmed: 26274468
Biochemistry. 2002 Nov 19;41(46):13473-82
pubmed: 12427007
J Phys Chem B. 2018 Nov 15;122(45):10291-10295
pubmed: 30339006
J Chem Phys. 2010 Jun 7;132(21):214508
pubmed: 20528032
J Phys Chem B. 2019 May 2;123(17):3679-3687
pubmed: 30964287
J Phys Chem B. 2010 Sep 23;114(37):12144-9
pubmed: 20799749
J Phys Chem B. 2016 Sep 8;120(35):9477-83
pubmed: 27526055
Phys Chem Chem Phys. 2015 May 7;17(17):11423-31
pubmed: 25853990
Faraday Discuss. 2013;167:177-90
pubmed: 24640491
J Phys Chem B. 2009 Jun 4;113(22):7874-8
pubmed: 19422249
Phys Rev Lett. 2005 Oct 7;95(15):158104
pubmed: 16241767
Biomacromolecules. 2021 Mar 8;22(3):1147-1158
pubmed: 33600168
Life (Basel). 2020 May 13;10(5):
pubmed: 32414105
J Phys Chem B. 2017 Sep 21;121(37):8731-8741
pubmed: 28829129
J Phys Chem Lett. 2017 Feb 2;8(3):611-614
pubmed: 28085291
J Am Chem Soc. 2011 Aug 10;133(31):12063-8
pubmed: 21699224
Sci Rep. 2013;3:1218
pubmed: 23390573
J Colloid Interface Sci. 2021 Dec 15;604:705-718
pubmed: 34280768
Annu Rev Biophys Biomol Struct. 1993;22:67-97
pubmed: 8347999
J Phys Chem B. 2012 Dec 27;116(51):14760-7
pubmed: 23205713
Phys Chem Chem Phys. 2016 Apr 7;18(13):8881-9
pubmed: 26958663
J Phys Chem B. 2012 Jun 28;116(25):7499-508
pubmed: 22651571
Nature. 2012 Nov 22;491(7425):582-5
pubmed: 23172216
Biophys J. 2006 Oct 1;91(7):2573-88
pubmed: 16844746
J Phys Chem Lett. 2018 Jan 4;9(1):120-125
pubmed: 29243934
J Am Chem Soc. 2008 Apr 9;130(14):4576-7
pubmed: 18336023
J Phys Chem Lett. 2017 Nov 2;8(21):5246-5252
pubmed: 28976760
J Chem Phys. 2019 Jul 7;151(1):015101
pubmed: 31272172
Science. 1984 Feb 17;223(4637):701-3
pubmed: 17841031
J Phys Chem B. 2010 Jun 24;114(24):8262-9
pubmed: 20509696
Int J Biol Macromol. 2021 Apr 30;177:40-47
pubmed: 33607130
Protein Sci. 2009 Jan;18(1):24-36
pubmed: 19177348
J Chem Phys. 2020 Dec 14;153(22):224503
pubmed: 33317300