Characterisation and preliminary functional analysis of N-acetyltransferase 13 from Schistosoma japonicum.
Acetyltransferases
/ genetics
Animals
Cloning, Molecular
DNA, Complementary
/ genetics
Female
Gene Expression Regulation, Enzymologic
/ physiology
Gene Silencing
Helminth Proteins
/ genetics
Male
Mice
Mice, Inbred BALB C
RNA, Messenger
Random Allocation
Schistosoma japonicum
/ enzymology
Schistosomiasis japonica
/ parasitology
Vaccines
/ immunology
Development
N-acetyltransferase
RNAi
Reproduction
Schistosoma japonicum
Schistosome
SjNAT13
Journal
BMC veterinary research
ISSN: 1746-6148
Titre abrégé: BMC Vet Res
Pays: England
ID NLM: 101249759
Informations de publication
Date de publication:
22 Oct 2021
22 Oct 2021
Historique:
received:
17
07
2021
accepted:
07
10
2021
entrez:
23
10
2021
pubmed:
24
10
2021
medline:
25
11
2021
Statut:
epublish
Résumé
N-acetyltransferase 13 (NAT13) is a probable catalytic component of the ARD1A-NARG1 complex possessing alpha (N-terminal) acetyltransferase activity. In this study, a full-length complementary DNA (cDNA) encoding Schistosoma japonicum NAT13 (SjNAT13) was isolated from schistosome cDNAs. The 621 bp open reading frame of SjNAT13 encodes a polypeptide of 206 amino acids. Real-time PCR analysis revealed SjNAT13 expression in all tested developmental stages. Transcript levels were highest in cercariae and 21-day-old worms, and higher in male adult worms than female adult worms. The rSjNAT13 protein induced high levels of anti-rSjNAT13 IgG antibodies. In two independent immunoprotection trials, rSjNAT13 induced 24.23% and 24.47% reductions in the numbers of eggs in liver. RNA interference (RNAi) results showed that small interfering RNA (siRNA) Sj-514 significantly reduced SjNAT13 transcript levels in worms and decreased egg production in vitro. Thus, rSjNAT13 might play an important role in the development and reproduction of schistosomes.
Sections du résumé
BACKGROUND
BACKGROUND
N-acetyltransferase 13 (NAT13) is a probable catalytic component of the ARD1A-NARG1 complex possessing alpha (N-terminal) acetyltransferase activity.
RESULTS
RESULTS
In this study, a full-length complementary DNA (cDNA) encoding Schistosoma japonicum NAT13 (SjNAT13) was isolated from schistosome cDNAs. The 621 bp open reading frame of SjNAT13 encodes a polypeptide of 206 amino acids. Real-time PCR analysis revealed SjNAT13 expression in all tested developmental stages. Transcript levels were highest in cercariae and 21-day-old worms, and higher in male adult worms than female adult worms. The rSjNAT13 protein induced high levels of anti-rSjNAT13 IgG antibodies. In two independent immunoprotection trials, rSjNAT13 induced 24.23% and 24.47% reductions in the numbers of eggs in liver. RNA interference (RNAi) results showed that small interfering RNA (siRNA) Sj-514 significantly reduced SjNAT13 transcript levels in worms and decreased egg production in vitro.
CONCLUSIONS
CONCLUSIONS
Thus, rSjNAT13 might play an important role in the development and reproduction of schistosomes.
Identifiants
pubmed: 34686208
doi: 10.1186/s12917-021-03045-y
pii: 10.1186/s12917-021-03045-y
pmc: PMC8540080
doi:
Substances chimiques
DNA, Complementary
0
Helminth Proteins
0
RNA, Messenger
0
Vaccines
0
Acetyltransferases
EC 2.3.1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
335Informations de copyright
© 2021. The Author(s).
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