Corticospinal neuron subpopulation-specific developmental genes prospectively indicate mature segmentally specific axon projection targeting.
Age Factors
Animals
Axons
/ metabolism
Bone Morphogenetic Protein Receptors
/ genetics
Cell Separation
Female
Flow Cytometry
Gene Expression Regulation, Developmental
Male
Mice, Inbred C57BL
Mice, Knockout
Nerve Tissue Proteins
/ genetics
Neuroanatomical Tract-Tracing Techniques
Neuronal Outgrowth
Pyramidal Tracts
/ growth & development
Sensorimotor Cortex
/ growth & development
Transcription, Genetic
White Matter
/ growth & development
bulbar-cervical
cortical development
cortical output circuitry
corticospinal axon guidance
corticospinal circuitry
corticospinal diversity
corticospinal tract
molecular controls over neuronal diversity
motor control
thoracolumbar
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
19 10 2021
19 10 2021
Historique:
received:
22
02
2017
revised:
27
05
2021
accepted:
26
09
2021
entrez:
23
10
2021
pubmed:
24
10
2021
medline:
16
2
2022
Statut:
ppublish
Résumé
For precise motor control, distinct subpopulations of corticospinal neurons (CSN) must extend axons to distinct spinal segments, from proximal targets in the brainstem and cervical cord to distal targets in thoracic and lumbar spinal segments. We find that developing CSN subpopulations exhibit striking axon targeting specificity in spinal white matter, which establishes the foundation for durable specificity of adult corticospinal circuitry. Employing developmental retrograde and anterograde labeling, and their distinct neocortical locations, we purified developing CSN subpopulations using fluorescence-activated cell sorting to identify genes differentially expressed between bulbar-cervical and thoracolumbar-projecting CSN subpopulations at critical developmental times. These segmentally distinct CSN subpopulations are molecularly distinct from the earliest stages of axon extension, enabling prospective identification even before eventual axon targeting decisions are evident in the spinal cord. This molecular delineation extends beyond simple spatial separation of these subpopulations in the cortex. Together, these results identify candidate molecular controls over segmentally specific corticospinal axon projection targeting.
Identifiants
pubmed: 34686320
pii: S2211-1247(21)01307-3
doi: 10.1016/j.celrep.2021.109843
pmc: PMC8653526
mid: NIHMS1749764
pii:
doi:
Substances chimiques
Crim1 protein, mouse
0
Klhl14 protein, mouse
0
Nerve Tissue Proteins
0
Bone Morphogenetic Protein Receptors
EC 2.7.11.30
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Video-Audio Media
Langues
eng
Sous-ensembles de citation
IM
Pagination
109843Subventions
Organisme : NINDS NIH HHS
ID : R01 NS049553
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS045523
Pays : United States
Organisme : NICHD NIH HHS
ID : K12 HD093427
Pays : United States
Organisme : NINDS NIH HHS
ID : F31 NS063516
Pays : United States
Organisme : NINDS NIH HHS
ID : F31 NS080343
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS104055
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS075672
Pays : United States
Organisme : NINDS NIH HHS
ID : DP1 NS106665
Pays : United States
Informations de copyright
Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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