Genetic deletion of platelet PAR4 results in reduced thrombosis and impaired hemostatic plug stability.

Animals Blood Platelets Hemostasis Hemostatics Mice Platelet Activation / physiology Platelet Aggregation Receptors, Thrombin / genetics Thrombin Thrombosis / genetics
hemostasis platelets protease-activated receptor 4 saphenous vein injury thrombosis

Journal

Journal of thrombosis and haemostasis : JTH
ISSN: 1538-7836
Titre abrégé: J Thromb Haemost
Pays: England
ID NLM: 101170508

Informations de publication

Date de publication:
02 2022
Historique:
revised: 19 10 2021
received: 26 06 2021
accepted: 20 10 2021
pubmed: 25 10 2021
medline: 16 3 2022
entrez: 24 10 2021
Statut: ppublish

Résumé

Protease-activated receptor 4 (PAR4) is expressed by a wide variety of cells, including megakaryocytes/platelets, immune cells, cardiomyocytes, and lung epithelial cells. It is the only functional thrombin receptor on murine platelets. A global deficiency of PAR4 is associated with impaired hemostasis and reduced thrombosis. We aimed to generate a mouse line with a megakaryocyte/platelet-specific deletion of PAR4 (PAR4 Platelets from PAR4 PAR4 We generated a novel PAR4

Sections du résumé

BACKGROUND
Protease-activated receptor 4 (PAR4) is expressed by a wide variety of cells, including megakaryocytes/platelets, immune cells, cardiomyocytes, and lung epithelial cells. It is the only functional thrombin receptor on murine platelets. A global deficiency of PAR4 is associated with impaired hemostasis and reduced thrombosis.
OBJECTIVE
We aimed to generate a mouse line with a megakaryocyte/platelet-specific deletion of PAR4 (PAR4
METHODS
Platelets from PAR4
RESULTS
PAR4
CONCLUSION
We generated a novel PAR4

Identifiants

DOI: 10.1111/jth.15569 PMID: 34689407 PMC: PMC8792346
pubmed: 34689407
doi: 10.1111/jth.15569
pmc: PMC8792346
mid: NIHMS1750788
pii: S1538-7836(22)02794-5
doi:

Substances chimiques

Hemostatics 0
Receptors, Thrombin 0
Thrombin EC 3.4.21.5
protease-activated receptor 4 JWE1M73YZN

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

422-433

Subventions

Organisme : NHLBI NIH HHS
ID : R01 HL142799
Pays : United States
Organisme : NHLBI NIH HHS
ID : R35 HL144976
Pays : United States

Informations de copyright

© 2021 International Society on Thrombosis and Haemostasis.

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Auteurs

Robert H Lee (RH)

UNC Blood Research Center, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
ORCID: 0000-0001-9871-0593

Tomohiro Kawano (T)

UNC Blood Research Center, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
ORCID: 0000-0002-6282-4991

Steven P Grover (SP)

UNC Blood Research Center, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.

Vanthana Bharathi (V)

UNC Blood Research Center, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.

David Martinez (D)

UNC Blood Research Center, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.

Dale O Cowley (DO)

UNC Animal Models Core, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.

Nigel Mackman (N)

UNC Blood Research Center, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
Division of Hematology, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
ORCID: 0000-0002-9170-7700

Wolfgang Bergmeier (W)

UNC Blood Research Center, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.

Silvio Antoniak (S)

UNC Blood Research Center, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
ORCID: 0000-0001-5523-825X

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Genetic deletion of platelet PAR4 results in...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Sang › Cellules sanguines › Plaquettes › Genetic deletion of platelet PAR4 results in...
questionsmedicales.fr › Phénomènes physiologiques respiratoires et circulatoires › Phénomènes physiogiques du sang › Hémostase › Genetic deletion of platelet PAR4 results in...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Agents hématologiques › Coagulants › Hémostatiques › Genetic deletion of platelet PAR4 results in...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Genetic deletion of platelet PAR4 results in...
questionsmedicales.fr › Phénomènes physiologiques respiratoires et circulatoires › Phénomènes physiogiques du sang › Hémostase › Activation plaquettaire › Genetic deletion of platelet PAR4 results in...
questionsmedicales.fr › Phénomènes physiologiques respiratoires et circulatoires › Phénomènes physiogiques du sang › Hémostase › Activation plaquettaire › Agrégation plaquettaire › Genetic deletion of platelet PAR4 results in...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Récepteurs activés par la protéinase › Récepteurs à la thrombine › Genetic deletion of platelet PAR4 results in...
questionsmedicales.fr › Facteurs biologiques › Facteurs de la coagulation sanguine › Thrombine › Genetic deletion of platelet PAR4 results in...
questionsmedicales.fr › Maladies cardiovasculaires › Maladies vasculaires › Embolie et thrombose › Thrombose › Genetic deletion of platelet PAR4 results in...