Influence of different cellular concentrations of boar sperm suspensions on the induction of capacitation and acrosome reaction.


Journal

The Journal of reproduction and development
ISSN: 1348-4400
Titre abrégé: J Reprod Dev
Pays: Japan
ID NLM: 9438792

Informations de publication

Date de publication:
18 Feb 2022
Historique:
pubmed: 26 10 2021
medline: 17 3 2022
entrez: 25 10 2021
Statut: ppublish

Résumé

We aimed to analyze the influence of different cellular concentrations of boar sperm suspensions on the induction of capacitation and acrosome reaction. When spermatozoa were incubated at 100 or 200 mill/ml, significant increases in protein tyrosine phosphorylation in the p32 protein were observed, compared to those at 50 mill/ml. In addition, sperm concentration-dependent increases were observed in plasma membrane lipid disorganization (50 mill/ml vs. 200 mill/ml), induction of the acrosome reaction (50 mill/ml vs. 100 mill/ml and 200 mill/ml), and sperm viability (50 mill/ml vs. 100 mill/ml and 200 mill/ml). Our data indicate that an increase in sperm concentration stimulates the induction of capacitation and acrosome reaction in boars.

Identifiants

pubmed: 34690211
doi: 10.1262/jrd.2021-075
pmc: PMC8872744
doi:

Substances chimiques

Suspensions 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

68-73

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Auteurs

David Martín-Hidalgo (D)

Research Group of Intracellular Signalling and Technology of Reproduction (Research Institute INBIO G+C), University of Extremadura, Cáceres, Spain.
Department of Biochemistry and Molecular Biology and Genetics, Faculty of Veterinary Sciences, University of Extremadura, Cáceres, Spain.

Beatriz Macías-García (B)

Research Group of Intracellular Signalling and Technology of Reproduction (Research Institute INBIO G+C), University of Extremadura, Cáceres, Spain.
Department of Animal Medicine, Faculty of Veterinary Sciences, University of Extremadura, Cáceres, Spain.

Lauro González-Fernández (L)

Research Group of Intracellular Signalling and Technology of Reproduction (Research Institute INBIO G+C), University of Extremadura, Cáceres, Spain.
Department of Biochemistry and Molecular Biology and Genetics, Faculty of Veterinary Sciences, University of Extremadura, Cáceres, Spain.

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Classifications MeSH