Free DNA and Metagenomics Analyses: Evaluation of Free DNA Inactivation Protocols for Shotgun Metagenomics Analysis of Human Biological Matrices.
free DNA
human microbiome
propidium monoazide
real-time PCR
shotgun metagenomics
Journal
Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977
Informations de publication
Date de publication:
2021
2021
Historique:
received:
29
07
2021
accepted:
09
09
2021
entrez:
25
10
2021
pubmed:
26
10
2021
medline:
26
10
2021
Statut:
epublish
Résumé
Culture-independent approaches now represent the gold standard for the investigation of both environmental and host-associated complex microbial communities. Nevertheless, despite the great advantages offered by these novel methodologies based on the use of next-generation DNA sequencing approaches, a number of bias sources have been identified. Among the latter, free DNA contained in biological matrices is one of the main sources of inaccuracy in reconstructing the resident microbial population of viable cells. For this reason, the photoreactive DNA-binding dye propidium monoazide (PMAxx
Identifiants
pubmed: 34691000
doi: 10.3389/fmicb.2021.749373
pmc: PMC8527314
doi:
Types de publication
Journal Article
Langues
eng
Pagination
749373Informations de copyright
Copyright © 2021 Mancabelli, Milani, Anzalone, Alessandri, Lugli, Tarracchini, Fontana, Turroni and Ventura.
Déclaration de conflit d'intérêts
RA was employed by the company GenProbio srl. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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