SLAMF7 selectively favors degranulation to promote cytotoxicity in human NK cells.
NK cells
SLAM receptors
SLAMF7
cell signaling
lymphocyte activation
Journal
European journal of immunology
ISSN: 1521-4141
Titre abrégé: Eur J Immunol
Pays: Germany
ID NLM: 1273201
Informations de publication
Date de publication:
01 2022
01 2022
Historique:
revised:
01
09
2021
received:
28
05
2021
accepted:
20
10
2021
pubmed:
26
10
2021
medline:
10
2
2022
entrez:
25
10
2021
Statut:
ppublish
Résumé
NK cells play an important role in immunity by recognizing and eliminating cells undergoing infection or malignant transformation. This role is dependent on the ability of NK cells to lyse targets cells in a perforin-dependent mechanism and by secreting inflammatory cytokines. Both effector functions are controlled by several cell surface receptors. The Signaling Lymphocyte Activation Molecule (SLAM) family of receptors plays an essential role in regulating NK cell activation. Several studies have demonstrated that SLAMF7 regulates NK cell activation. However, the molecular and cellular mechanisms by which SLAMF7 influences NK effector functions are unknown. Here, we present evidence that physiological ligation of SLAMF7 in human NK cells enhances the lysis of target cells expressing SLAMF7. This effect was dependent on the ability of SLAMF7 to promote NK cell degranulation rather than cytotoxic granule polarization or cell adhesion. Moreover, SLAMF7-dependent NK cell degranulation was predominantly dependent on PLC-γ when compared to PI3K. These data provide novel information on the cellular mechanism by which SLAMF7 regulates human NK cell activation. Finally, this study supports a model for NK cell activation where activated receptors contribute by regulating specific discrete cellular events rather than multiple cellular processes.
Identifiants
pubmed: 34693521
doi: 10.1002/eji.202149406
doi:
Substances chimiques
SLAMF7 protein, human
0
Signaling Lymphocytic Activation Molecule Family
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
62-74Subventions
Organisme : CIHR
Pays : Canada
Informations de copyright
© 2021 Wiley-VCH GmbH.
Références
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