Enemy of My Enemy: A Novel Insect-Specific Flavivirus Offers a Promising Platform for a Zika Virus Vaccine.

Zika virus correlates of protection flavivirus insect-specific virus vaccine

Journal

Vaccines
ISSN: 2076-393X
Titre abrégé: Vaccines (Basel)
Pays: Switzerland
ID NLM: 101629355

Informations de publication

Date de publication:
07 Oct 2021
Historique:
received: 25 08 2021
revised: 25 09 2021
accepted: 30 09 2021
entrez: 26 10 2021
pubmed: 27 10 2021
medline: 27 10 2021
Statut: epublish

Résumé

Vaccination remains critical for viral disease outbreak prevention and control, but conventional vaccine development typically involves trade-offs between safety and immunogenicity. We used a recently discovered insect-specific flavivirus as a vector in order to develop an exceptionally safe, flavivirus vaccine candidate with single-dose efficacy. To evaluate the safety and efficacy of this platform, we created a chimeric Zika virus (ZIKV) vaccine candidate, designated Aripo/Zika virus (ARPV/ZIKV). ZIKV has caused immense economic and public health impacts throughout the Americas and remains a significant public health threat. ARPV/ZIKV vaccination showed exceptional safety due to ARPV/ZIKV's inherent vertebrate host-restriction. ARPV/ZIKV showed no evidence of replication or translation in vitro and showed no hematological, histological or pathogenic effects in vivo. A single-dose immunization with ARPV/ZIKV induced rapid and robust neutralizing antibody and cellular responses, which offered complete protection against ZIKV-induced morbidity, mortality and in utero transmission in immune-competent and -compromised murine models. Splenocytes derived from vaccinated mice demonstrated significant CD4

Identifiants

pubmed: 34696250
pii: vaccines9101142
doi: 10.3390/vaccines9101142
pmc: PMC8539214
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : NIAID NIH HHS
ID : K22 AI125474
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI153433
Pays : United States
Organisme : NIH HHS
ID : K22AI125474; R01AI153433; R03AI151494; R24AI120942
Pays : United States
Organisme : NIAID NIH HHS
ID : R03 AI151494
Pays : United States
Organisme : NIAID NIH HHS
ID : R24 AI120942
Pays : United States

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Auteurs

Danielle L Porier (DL)

Department of Entomology, Fralin Life Science Institute, Virginia Tech, Blacksburg, VA 24061, USA.

Sarah N Wilson (SN)

Department of Entomology, Fralin Life Science Institute, Virginia Tech, Blacksburg, VA 24061, USA.

Dawn I Auguste (DI)

Department of Entomology, Fralin Life Science Institute, Virginia Tech, Blacksburg, VA 24061, USA.

Andrew Leber (A)

Nutritional Immunology and Molecular Medicine Laboratory Institute, Blacksburg, VA 24060, USA.

Sheryl Coutermarsh-Ott (S)

Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Blacksburg, VA 24060, USA.

Irving C Allen (IC)

Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Blacksburg, VA 24060, USA.
Center for Emerging, Zoonotic, and Arthropod-Borne Pathogens, Virginia Tech, Blacksburg, VA 24061, USA.

Clayton C Caswell (CC)

Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Blacksburg, VA 24060, USA.
Center for One Health Research, Virginia-Maryland College of Veterinary Medicine, Blacksburg, VA 24060, USA.

James A Budnick (JA)

Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Blacksburg, VA 24060, USA.
Center for One Health Research, Virginia-Maryland College of Veterinary Medicine, Blacksburg, VA 24060, USA.

Josep Bassaganya-Riera (J)

Nutritional Immunology and Molecular Medicine Laboratory Institute, Blacksburg, VA 24060, USA.

Raquel Hontecillas (R)

Nutritional Immunology and Molecular Medicine Laboratory Institute, Blacksburg, VA 24060, USA.

James Weger-Lucarelli (J)

Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Blacksburg, VA 24060, USA.
Center for Emerging, Zoonotic, and Arthropod-Borne Pathogens, Virginia Tech, Blacksburg, VA 24061, USA.

Scott C Weaver (SC)

World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch, Galveston, TX 77555, USA.
Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX 77555, USA.
Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA.

Albert J Auguste (AJ)

Department of Entomology, Fralin Life Science Institute, Virginia Tech, Blacksburg, VA 24061, USA.
Center for Emerging, Zoonotic, and Arthropod-Borne Pathogens, Virginia Tech, Blacksburg, VA 24061, USA.

Classifications MeSH