Gag-Gag Interactions Are Insufficient to Fully Stabilize and Order the Immature HIV Gag Lattice.


Journal

Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722

Informations de publication

Date de publication:
28 09 2021
Historique:
received: 01 09 2021
revised: 24 09 2021
accepted: 24 09 2021
entrez: 26 10 2021
pubmed: 27 10 2021
medline: 10 2 2022
Statut: epublish

Résumé

Immature HIV virions harbor a lattice of Gag molecules with significant ordering in CA-NTD, CA-CTD and SP1 regions. This ordering plays a major role during HIV maturation. To test the condition in which the Gag lattice forms in vivo, we assembled virus like particles (VLPs) by expressing only HIV Gag in mammalian cells. Here we show that these VLPs incorporate a similar number of Gag molecules compared to immature HIV virions. However, within these VLPs, Gag molecules diffuse with a pseudo-diffusion rate of 10 nm

Identifiants

pubmed: 34696376
pii: v13101946
doi: 10.3390/v13101946
pmc: PMC8540168
pii:
doi:

Substances chimiques

gag Gene Products, Human Immunodeficiency Virus 0

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NIAID NIH HHS
ID : R01 AI150474
Pays : United States
Organisme : NIH HHS
ID : R01 AI150474
Pays : United States

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Auteurs

Ipsita Saha (I)

Laboratory of Cell and Developmental Signaling, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA.

Benjamin Preece (B)

Center for Cell and Genome Science, University of Utah, Salt Lake City, UT 84112, USA.
Department of Physics and Astronomy, University of Utah, Salt Lake City, UT 84112, USA.

Abby Peterson (A)

Center for Cell and Genome Science, University of Utah, Salt Lake City, UT 84112, USA.
Department of Physics and Astronomy, University of Utah, Salt Lake City, UT 84112, USA.

Haley Durden (H)

Center for Cell and Genome Science, University of Utah, Salt Lake City, UT 84112, USA.
Department of Physics and Astronomy, University of Utah, Salt Lake City, UT 84112, USA.

Brian MacArthur (B)

Center for Cell and Genome Science, University of Utah, Salt Lake City, UT 84112, USA.
Department of Physics and Astronomy, University of Utah, Salt Lake City, UT 84112, USA.

Jake Lowe (J)

School of Biological Sciences, University of Utah, Salt Lake City, UT 84112, USA.

David Belnap (D)

School of Biological Sciences, University of Utah, Salt Lake City, UT 84112, USA.
Department of Biochemistry, University of Utah, Salt Lake City, UT 84112, USA.

Michael Vershinin (M)

Center for Cell and Genome Science, University of Utah, Salt Lake City, UT 84112, USA.
Department of Physics and Astronomy, University of Utah, Salt Lake City, UT 84112, USA.
School of Biological Sciences, University of Utah, Salt Lake City, UT 84112, USA.

Saveez Saffarian (S)

Center for Cell and Genome Science, University of Utah, Salt Lake City, UT 84112, USA.
Department of Physics and Astronomy, University of Utah, Salt Lake City, UT 84112, USA.
School of Biological Sciences, University of Utah, Salt Lake City, UT 84112, USA.

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