Mitochondrial phenotypes in purified human immune cell subtypes and cell mixtures.
aging
cell biology
dynamic variation
human
immunology
immunometabolism
inflammation
leukocytes
mitochondria
mitotypes
sexual dimorphism
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
26 10 2021
26 10 2021
Historique:
received:
02
06
2021
accepted:
26
10
2021
pubmed:
27
10
2021
medline:
27
1
2022
entrez:
26
10
2021
Statut:
epublish
Résumé
Using a high-throughput mitochondrial phenotyping platform to quantify multiple mitochondrial features among molecularly defined immune cell subtypes, we quantify the natural variation in mitochondrial DNA copy number (mtDNAcn), citrate synthase, and respiratory chain enzymatic activities in human neutrophils, monocytes, B cells, and naïve and memory T lymphocyte subtypes. In mixed peripheral blood mononuclear cells (PBMCs) from the same individuals, we show to what extent mitochondrial measures are confounded by both cell type distributions and contaminating platelets. Cell subtype-specific measures among women and men spanning four decades of life indicate potential age- and sex-related differences, including an age-related elevation in mtDNAcn, which are masked or blunted in mixed PBMCs. Finally, a proof-of-concept, repeated-measures study in a single individual validates cell type differences and also reveals week-to-week changes in mitochondrial activities. Larger studies are required to validate and mechanistically extend these findings. These mitochondrial phenotyping data build upon established immunometabolic differences among leukocyte subpopulations, and provide foundational quantitative knowledge to develop interpretable blood-based assays of mitochondrial health.
Identifiants
pubmed: 34698636
doi: 10.7554/eLife.70899
pii: 70899
pmc: PMC8612706
doi:
pii:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIA NIH HHS
ID : R01 AG066828
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM119793
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH119336
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH122706
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA013696
Pays : United States
Organisme : NIA NIH HHS
ID : R01 AG026307
Pays : United States
Organisme : NIA NIH HHS
ID : K99 AG056635
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR001873
Pays : United States
Organisme : NIA NIH HHS
ID : R00 AG056635
Pays : United States
Informations de copyright
© 2021, Rausser et al.
Déclaration de conflit d'intérêts
SR, CT, MM, AJ, WW, SH, AM, KK, CM, SS, RR, MP No competing interests declared
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