Generation and characterization of novel co-stimulatory anti-mouse TNFR2 antibodies.
Antibody
Costimulation
Cysteine-rich domain
Epitope
TNFR2
Treg
Journal
Journal of immunological methods
ISSN: 1872-7905
Titre abrégé: J Immunol Methods
Pays: Netherlands
ID NLM: 1305440
Informations de publication
Date de publication:
12 2021
12 2021
Historique:
received:
03
09
2021
revised:
18
10
2021
accepted:
19
10
2021
pubmed:
27
10
2021
medline:
30
11
2021
entrez:
26
10
2021
Statut:
ppublish
Résumé
Tumor necrosis factor receptor 2 (TNFR2) has gained much research interest in recent years because of its potential pivotal role in autoimmune disease and cancer. However, its function in regulating different immune cells is not well understood. There is a need for well-characterized reagents to selectively modulate TNFR2 function, thereby enabling definition of TNFR2-dependent biology in human and mouse surrogate models. Here, we describe the generation, production, purification, and characterization of a panel of novel antibodies targeting mouse TNFR2. The antibodies display functional differences in binding affinity and potency to block TNFα. Furthermore, epitope binding showed that the anti-mTNFR2 antibodies target different domains on the TNFR2 protein, associated with varying capacity to enhance CD8
Identifiants
pubmed: 34699840
pii: S0022-1759(21)00218-0
doi: 10.1016/j.jim.2021.113173
pii:
doi:
Substances chimiques
Antibodies
0
Receptors, Tumor Necrosis Factor, Type II
0
Tnfrsf1b protein, mouse
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
113173Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.