Effects of Varied Stimulation Parameters on Adipose-Derived Stem Cell Response to Low-Level Electrical Fields.
Cell-based therapies
Frequency
Growth factor
Neural regeneration
Neurite outgrowth
SH-SY5Y
Schwann cell
Secretome
Journal
Annals of biomedical engineering
ISSN: 1573-9686
Titre abrégé: Ann Biomed Eng
Pays: United States
ID NLM: 0361512
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
received:
05
06
2021
accepted:
04
10
2021
pubmed:
28
10
2021
medline:
23
3
2022
entrez:
27
10
2021
Statut:
ppublish
Résumé
Exogenous electrical fields have been explored in regenerative medicine to increase cellular expression of pro-regenerative growth factors. Adipose-derived stem cells (ASCs) are attractive for regenerative applications, specifically for neural repair. Little is known about the relationship between low-level electrical stimulation (ES) and ASC regenerative potentiation. In this work, patterns of ASC expression and secretion of growth factors (i.e., secretome) were explored across a range of ES parameters. ASCs were stimulated with low-level stimulation (20 mV/mm) at varied pulse frequencies, durations, and with alternating versus direct current. Frequency and duration had the most significant effects on growth factor expression. While a range of stimulation frequencies (1, 20, 1000 Hz) applied intermittently (1 h × 3 days) induced upregulation of general wound healing factors, neural-specific factors were only increased at 1 Hz. Moreover, the most optimal expression of neural growth factors was achieved when ASCs were exposed to 1 Hz pulses continuously for 24 h. In evaluation of secretome, apparent inconsistencies were observed across biological replications. Nonetheless, ASC secretome (from 1 Hz, 24 h ES) caused significant increase in neurite extension compared to non-stimulated control. Overall, ASCs are sensitive to ES parameters at low field strengths, notably pulse frequency and stimulation duration.
Identifiants
pubmed: 34704163
doi: 10.1007/s10439-021-02875-z
pii: 10.1007/s10439-021-02875-z
pmc: PMC10947800
mid: NIHMS1809507
doi:
Substances chimiques
Nerve Growth Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3401-3411Subventions
Organisme : NINDS NIH HHS
ID : R21 NS111398
Pays : United States
Informations de copyright
© 2021. Biomedical Engineering Society.
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