Assaying Cyclin-Dependent Kinase Activity in Synchronized Algal Cultures.
Cell division
Commitment point
DNA replication
Green algae
Kinase activity
Multiple fission cell cycle
Nuclear division
Synchronization
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
27
10
2021
pubmed:
28
10
2021
medline:
5
1
2022
Statut:
ppublish
Résumé
Cyclin-dependent kinases (CDKs) are key regulators of the cell cycle in eukaryotes. Assessing their activity is one of the basic methods used to analyze their function. This is particularly true in synchronized cultures of unicellular organisms, where the entire culture is in the same physiological state. In this chapter, I describe a simple biochemical method to assess CDK activity in algae. Although the results are easier to interpret in the context of synchronized cultures, the method is not limited to them. The protocol requires only standard laboratory equipment and access to a radioactivity working room. The method is applicable to any algal species, including newly developed ones, as it does not require any specific tools. The method can, therefore, be used to widen the portfolio of cell cycle regulatory models within algae.
Identifiants
pubmed: 34705233
doi: 10.1007/978-1-0716-1744-1_4
doi:
Substances chimiques
Cell Cycle Proteins
0
Cyclin-Dependent Kinase 2
EC 2.7.11.22
Cyclin-Dependent Kinases
EC 2.7.11.22
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
73-88Informations de copyright
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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