Analyses of Protein Turnover at the Cell Plate by Fluorescence Recovery After Photobleaching During Cytokinesis.

Cytokinesis Cytoplasm Fluorescence Recovery After Photobleaching
Cell plate Cytokinesis FRAP Fluorescence recovery Syntaxins

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2022
Historique:
entrez: 27 10 2021
pubmed: 28 10 2021
medline: 5 1 2022
Statut: ppublish

Résumé

Membrane trafficking is central to cell plate construction during plant cytokinesis. Studies on cell plate formation can provide answers to basic biology questions including molecular mechanisms of membrane trafficking, tissue patterning, and cytoskeletal dynamics. Consequently, a detailed understanding of cytokinesis depends on the characterization of molecules that function in the formation, transport, targeting, and fusion of membrane vesicles and delivery of proteins to the developing and maturing plate. This chapter describes a pipeline based on fluorescence recovery after photobleaching (FRAP) to measure and analyze turnover of peripheral or transmembrane proteins on the cell plate. The approach described here can also be applied in other biological contexts.

Identifiants

DOI: 10.1007/978-1-0716-1744-1_14 PMID: 34705243
pubmed: 34705243
doi: 10.1007/978-1-0716-1744-1_14
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

233-243

Informations de copyright

© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Références

Livanos P, Müller S (2019) Division plane establishment and cytokinesis. Annu Rev Plant Biol 70(1):239–267
doi: 10.1146/annurev-arplant-050718-100444
Moschou PN, Smertenko AP, Minina EA et al (2013) The caspase-related protease separase (extra spindle poles) regulates cell polarity and cytokinesis in Arabidopsis. Plant Cell 25(6):2171–2186
doi: 10.1105/tpc.113.113043
El Kasmi F, Krause C, Hiller U et al (2013) SNARE complexes of different composition jointly mediate membrane fusion in Arabidopsis cytokinesis. Mol Biol Cell 24(10):1593–1601
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doi: 10.1038/35103000
Boutte Y, Frescatada-Rosa M, Men S et al (2010) Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis. EMBO J 29(3):546–558
doi: 10.1038/emboj.2009.363
Moschou PN, Gutierrez-Beltran E, Bozhkov PV, Smertenko A (2016) Separase promotes microtubule polymerization by activating CENP-E-related kinesin Kin7. Dev Cell 37(4):350–361
doi: 10.1016/j.devcel.2016.04.015
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Auteurs

Alexandra Deli (A)

Department of Biology, University of Crete, Heraklion, Greece.
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Heraklion, Greece.

Leda-Eleni Tympa (LE)

Department of Biology, University of Crete, Heraklion, Greece.
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Heraklion, Greece.

Panagiotis N Moschou (PN)

Department of Biology, University of Crete, Heraklion, Greece. Panagiotis.moschou@imbb.forth.gr.
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Heraklion, Greece. Panagiotis.moschou@imbb.forth.gr.
Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, Uppsala, Sweden. Panagiotis.moschou@imbb.forth.gr.

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Classifications MeSH

questionsmedicales.fr › Phénomènes physiologiques cellulaires › Cycle cellulaire › Cytocinèse › Analyses of Protein Turnover at the Cell Plate...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Analyses of Protein Turnover at the Cell Plate...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Photométrie › Mesures de luminescence › Fluorimétrie › Redistribution de fluorescence après photoblanchiment › Analyses of Protein Turnover at the Cell Plate...