Mechanical behaviour of healthy versus alkali-lesioned corneas by a porcine organ culture model.
Alkali-induced lesions
Biomechanical behaviour
Porcine cornea
Riboflavin/UV-A corneal phototherapy
Statistical analysis
Tensile tests
Journal
BMC veterinary research
ISSN: 1746-6148
Titre abrégé: BMC Vet Res
Pays: England
ID NLM: 101249759
Informations de publication
Date de publication:
28 Oct 2021
28 Oct 2021
Historique:
received:
03
05
2021
accepted:
24
09
2021
entrez:
29
10
2021
pubmed:
30
10
2021
medline:
25
11
2021
Statut:
epublish
Résumé
Cornea is a composite tissue exhibiting nonlinear and time-dependent mechanical properties. Corneal ulcers are one of the main pathologies that affect this tissue, disrupting its structural integrity and leading to impaired functions. In this study, uniaxial tensile and stress-relaxation tests are developed to evaluate stress-strain and time-dependent mechanical behaviour of porcine corneas. The samples are split in two groups: some corneas are analysed in an unaltered state (healthy samples), while others are injured with alkaline solution to create an experimental ulcer (lesioned samples). Furthermore, within each group, corneas are examined in two conditions: few hours after the enucleation (fresh samples) or after 7 days in a specific culture medium for the tissue (cultured samples). Finally, another condition is added: corneas from all the groups undergo or not a cross-linking treatment. In both stress-strain and stress-relaxation tests, a weakening of the tissue is observed due to the imposed conditions (lesion, culture and treatment), represented by a lower stiffness and increased stress-relaxation. Alkali-induced corneal stromal melting determines changes in the mechanical response that can be related to a damage at microstructural level. The results of the present study represent the basis for the investigation of traditional and innovative corneal therapies.
Sections du résumé
BACKGROUND
BACKGROUND
Cornea is a composite tissue exhibiting nonlinear and time-dependent mechanical properties. Corneal ulcers are one of the main pathologies that affect this tissue, disrupting its structural integrity and leading to impaired functions. In this study, uniaxial tensile and stress-relaxation tests are developed to evaluate stress-strain and time-dependent mechanical behaviour of porcine corneas.
RESULTS
RESULTS
The samples are split in two groups: some corneas are analysed in an unaltered state (healthy samples), while others are injured with alkaline solution to create an experimental ulcer (lesioned samples). Furthermore, within each group, corneas are examined in two conditions: few hours after the enucleation (fresh samples) or after 7 days in a specific culture medium for the tissue (cultured samples). Finally, another condition is added: corneas from all the groups undergo or not a cross-linking treatment. In both stress-strain and stress-relaxation tests, a weakening of the tissue is observed due to the imposed conditions (lesion, culture and treatment), represented by a lower stiffness and increased stress-relaxation.
CONCLUSIONS
CONCLUSIONS
Alkali-induced corneal stromal melting determines changes in the mechanical response that can be related to a damage at microstructural level. The results of the present study represent the basis for the investigation of traditional and innovative corneal therapies.
Identifiants
pubmed: 34711207
doi: 10.1186/s12917-021-03050-1
pii: 10.1186/s12917-021-03050-1
pmc: PMC8555156
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
340Informations de copyright
© 2021. The Author(s).
Références
Exp Eye Res. 2007 Sep;85(3):305-11
pubmed: 17655845
J Appl Biomater Funct Mater. 2016 May 18;14(2):e163-70
pubmed: 26952586
Exp Eye Res. 2006 Oct;83(4):709-20
pubmed: 16720023
J Cell Sci. 2008 Oct 15;121(Pt 20):3285-92
pubmed: 18843115
J Mech Behav Biomed Mater. 2020 Oct;110:103883
pubmed: 32957190
J Refract Surg. 2018 Sep 1;34(9):622-627
pubmed: 30199567
Exp Eye Res. 2016 Jan;142:110-8
pubmed: 26675407
Animals (Basel). 2020 Apr 23;10(4):
pubmed: 32340101
J R Soc Interface. 2005 Jun 22;2(3):177-85
pubmed: 16849178
Refract Corneal Surg. 1992 Mar-Apr;8(2):139-42
pubmed: 1591208
Biophys Rev. 2009 Jul;1(2):83-93
pubmed: 28509987
Acta Biomater. 2015 Jan;11:324-32
pubmed: 25305512
Vet Ophthalmol. 2013 Nov;16(6):464-6
pubmed: 23437914
Invest Ophthalmol Vis Sci. 2010 Aug;51(8):3961-8
pubmed: 20335615
Vet Ophthalmol. 2014 Jul;17(4):250-60
pubmed: 23941330
Invest Ophthalmol Vis Sci. 2013 Feb 19;54(2):1418-25
pubmed: 23361513
Exp Eye Res. 2008 May;86(5):783-90
pubmed: 18396276
J Cataract Refract Surg. 2003 Sep;29(9):1780-5
pubmed: 14522301
Cornea. 2017 Jul;36(7):854-859
pubmed: 28486312
Rom J Morphol Embryol. 2013;54(4):1115-20
pubmed: 24399010
Arch Ophthalmol. 2006 Apr;124(4):471-6
pubmed: 16606871
Biophys J. 2000 Jul;79(1):144-52
pubmed: 10866943
Invest Ophthalmol Vis Sci. 2009 Nov;50(11):5148-54
pubmed: 19516014
BMC Vet Res. 2013 Jun 26;9:128
pubmed: 23803176
Turk J Ophthalmol. 2018 Aug;48(4):160-165
pubmed: 30202610
J Biomech. 2001 Apr;34(4):533-7
pubmed: 11266678
Ocul Surf. 2013 Apr;11(2):65-74
pubmed: 23583042
Eye Vis (Lond). 2016 Aug 10;3:21
pubmed: 27512719
J Refract Surg. 2017 Mar 1;33(3):184-192
pubmed: 28264133
Invest Ophthalmol Vis Sci. 2016 Jul 1;57(9):OCT112-20
pubmed: 27409461
Exp Physiol. 2016 May 1;101(5):641-56
pubmed: 26864993
J Biomech. 2017 May 3;56:32-41
pubmed: 28314563
Vet Ophthalmol. 2014 Jan;17(1):1-11
pubmed: 23356663
Surv Ophthalmol. 2018 Nov - Dec;63(6):851-861
pubmed: 29857022
Am J Ophthalmol. 2003 Sep;136(3):530-6
pubmed: 12967809
Vet Ophthalmol. 2014 Sep;17(5):358-67
pubmed: 23902524
J Biomech Eng. 2012 Mar;134(3):031003
pubmed: 22482683