A SARS-CoV-2 spike ferritin nanoparticle vaccine protects hamsters against Alpha and Beta virus variant challenge.

Journal

NPJ vaccines
ISSN: 2059-0105
Titre abrégé: NPJ Vaccines
Pays: England
ID NLM: 101699863

Informations de publication

Date de publication:
28 Oct 2021
Historique:
received: 16 06 2021
accepted: 23 09 2021
entrez: 29 10 2021
pubmed: 30 10 2021
medline: 30 10 2021
Statut: epublish

Résumé

The emergence of SARS-CoV-2 variants of concern (VOC) requires adequate coverage of vaccine protection. We evaluated whether a SARS-CoV-2 spike ferritin nanoparticle vaccine (SpFN), adjuvanted with the Army Liposomal Formulation QS21 (ALFQ), conferred protection against the Alpha (B.1.1.7), and Beta (B.1.351) VOCs in Syrian golden hamsters. SpFN-ALFQ was administered as either single or double-vaccination (0 and 4 week) regimens, using a high (10 μg) or low (0.2 μg) dose. Animals were intranasally challenged at week 11. Binding antibody responses were comparable between high- and low-dose groups. Neutralizing antibody titers were equivalent against WA1, B.1.1.7, and B.1.351 variants following two high dose vaccinations. Dose-dependent SpFN-ALFQ vaccination protected against SARS-CoV-2-induced disease and viral replication following intranasal B.1.1.7 or B.1.351 challenge, as evidenced by reduced weight loss, lung pathology, and lung and nasal turbinate viral burden. These data support the development of SpFN-ALFQ as a broadly protective, next-generation SARS-CoV-2 vaccine.

Identifiants

DOI: 10.1038/s41541-021-00392-7 PMID: 34711815 PMC: PMC8553838
pubmed: 34711815
doi: 10.1038/s41541-021-00392-7
pii: 10.1038/s41541-021-00392-7
pmc: PMC8553838
doi:

Types de publication

Journal Article

Langues

eng

Pagination

129

Commentaires et corrections

Type : UpdateOf

Informations de copyright

© 2021. This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply.

Références

Expert Rev Vaccines. 2020 Mar;19(3):279-292
pubmed: 32228108
N Engl J Med. 2021 Jun 10;384(23):2212-2218
pubmed: 33882219
Clin Infect Dis. 2020 Dec 3;71(9):2428-2446
pubmed: 32215622
Nature. 2021 Feb;590(7844):E25
pubmed: 33469218
Nature. 2020 Jul;583(7818):834-838
pubmed: 32408338
J Exp Med. 2021 Mar 1;218(3):
pubmed: 33211088
Nat Med. 2020 Nov;26(11):1694-1700
pubmed: 32884153
Nat Commun. 2021 Jun 30;12(1):4048
pubmed: 34193869
Science. 2020 Aug 14;369(6505):806-811
pubmed: 32434945
Cell. 2020 Apr 16;181(2):271-280.e8
pubmed: 32142651
Nature. 2021 May;593(7857):130-135
pubmed: 33684923
MMWR Morb Mortal Wkly Rep. 2021 Jan 22;70(3):95-99
pubmed: 33476315
Cell. 2021 Apr 29;184(9):2372-2383.e9
pubmed: 33743213
N Engl J Med. 2020 Oct 15;383(16):1544-1555
pubmed: 32722908
Nature. 2021 Apr;592(7854):438-443
pubmed: 33690265
Nature. 2020 Oct;586(7830):509-515
pubmed: 32967005
Nat Med. 2021 Apr;27(4):622-625
pubmed: 33654292
Proc Natl Acad Sci U S A. 2021 Sep 21;118(38):
pubmed: 34470866
Cell. 2021 Apr 1;184(7):1804-1820.e16
pubmed: 33691139
Cell. 2021 Apr 29;184(9):2316-2331.e15
pubmed: 33773105
Nat Commun. 2021 Oct 7;12(1):5868
pubmed: 34620866
Proc Natl Acad Sci U S A. 2020 Jul 14;117(28):16587-16595
pubmed: 32571934
Cell Mol Immunol. 2021 Apr;18(4):1061-1063
pubmed: 33633321
Nature. 2020 Oct;586(7830):583-588
pubmed: 32731257
Nat Rev Microbiol. 2021 Mar;19(3):141-154
pubmed: 33024307

Auteurs

Kathryn McGuckin Wuertz (KM)

U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.

Erica K Barkei (EK)

Veterinary Pathology Division, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
ORCID: 0000-0003-1120-7894

Wei-Hung Chen (WH)

Emerging Infectious Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
ORCID: 0000-0003-3724-9195

Elizabeth J Martinez (EJ)

Emerging Infectious Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
ORCID: 0000-0002-7755-7056

Ines Lakhal-Naouar (I)

Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
Diagnostics Countermeasures Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.

Linda L Jagodzinski (LL)

Diagnostics Countermeasures Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.

Dominic Paquin-Proulx (D)

U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
ORCID: 0000-0003-1407-3414

Gregory D Gromowski (GD)

Virus Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
ORCID: 0000-0003-4576-4277

Isabella Swafford (I)

U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.

Akshaya Ganesh (A)

U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Oak Ridge Institute of Science and Education, Oak Ridge, TN, USA.

Ming Dong (M)

U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.

Xiankun Zeng (X)

Pathology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD, USA.

Paul V Thomas (PV)

Emerging Infectious Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.

Rajeshwer S Sankhala (RS)

Emerging Infectious Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.

Agnes Hajduczki (A)

Emerging Infectious Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.

Caroline E Peterson (CE)

Emerging Infectious Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.

Caitlin Kuklis (C)

Virus Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.

Sandrine Soman (S)

Virus Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.

Lindsay Wieczorek (L)

U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.

Michelle Zemil (M)

U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
ORCID: 0000-0002-1700-6658

Alexander Anderson (A)

Emerging Infectious Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Oak Ridge Institute of Science and Education, Oak Ridge, TN, USA.
ORCID: 0000-0002-0000-7734

Janice Darden (J)

Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
Diagnostics Countermeasures Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.

Heather Hernandez (H)

Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
Diagnostics Countermeasures Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.

Hannah Grove (H)

Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
Diagnostics Countermeasures Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.

Vincent Dussupt (V)

U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
ORCID: 0000-0002-8579-6356

Holly Hack (H)

Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
Diagnostics Countermeasures Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.

Rafael de la Barrera (R)

Pilot Bioproduction Facility, Walter Reed Army Institute of Research, Silver Spring, MD, USA.

Stasya Zarling (S)

Pilot Bioproduction Facility, Walter Reed Army Institute of Research, Silver Spring, MD, USA.

James F Wood (JF)

Pilot Bioproduction Facility, Walter Reed Army Institute of Research, Silver Spring, MD, USA.

Jeffrey W Froude (JW)

Pilot Bioproduction Facility, Walter Reed Army Institute of Research, Silver Spring, MD, USA.

Matthew Gagne (M)

Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Amy R Henry (AR)

Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Elham Bayat Mokhtari (EB)

Virus Persistence and Dynamics Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Prakriti Mudvari (P)

Virus Persistence and Dynamics Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Shelly J Krebs (SJ)

U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
ORCID: 0000-0003-1136-1760

Andrew S Pekosz (AS)

W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.
ORCID: 0000-0003-3248-1761

Jeffrey R Currier (JR)

Virus Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
ORCID: 0000-0002-5696-0268

Swagata Kar (S)

BioQual, Inc., Rockville, MD, USA.

Maciel Porto (M)

BioQual, Inc., Rockville, MD, USA.
ORCID: 0000-0002-3427-454X

Adrienne Winn (A)

BioQual, Inc., Rockville, MD, USA.

Kamil Radzyminski (K)

BioQual, Inc., Rockville, MD, USA.

Mark G Lewis (MG)

BioQual, Inc., Rockville, MD, USA.
ORCID: 0000-0001-7852-0135

Sandhya Vasan (S)

Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
ORCID: 0000-0002-6378-6288

Mehul Suthar (M)

Emory Vaccine Center, Department of Pediatrics, Emory School of Medicine, Atlanta, GA, USA.

Victoria R Polonis (VR)

U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.

Gary R Matyas (GR)

U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.

Eli A Boritz (EA)

Virus Persistence and Dynamics Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Daniel C Douek (DC)

Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.

Robert A Seder (RA)

Cellular Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
ORCID: 0000-0003-3133-0849

Sharon P Daye (SP)

One Health Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.

Mangala Rao (M)

U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.

Sheila A Peel (SA)

Diagnostics Countermeasures Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.

M Gordon Joyce (MG)

Emerging Infectious Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
ORCID: 0000-0002-6808-7232

Diane L Bolton (DL)

U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.
ORCID: 0000-0003-1897-499X

Nelson L Michael (NL)

Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA. nelson.l.michael2.civ@mail.mil.
ORCID: 0000-0001-5882-5548

Kayvon Modjarrad (K)

Emerging Infectious Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA. kayvon.modjarrad.civ@mail.mil.

Classifications MeSH