Multiallelic, Targeted Mutagenesis of Magnesium Chelatase With CRISPR/Cas9 Provides a Rapidly Scorable Phenotype in Highly Polyploid Sugarcane.

CRISPR/Cas9 biolistic gene transfer genome editing heat treatment magnesium chelatase polyploid sugarcane

Journal

Frontiers in genome editing
ISSN: 2673-3439
Titre abrégé: Front Genome Ed
Pays: Switzerland
ID NLM: 101775540

Informations de publication

Date de publication:
2021
Historique:
received: 18 01 2021
accepted: 15 03 2021
entrez: 29 10 2021
pubmed: 30 10 2021
medline: 30 10 2021
Statut: epublish

Résumé

Genome editing with sequence-specific nucleases, such as clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9), is revolutionizing crop improvement. Developing efficient genome-editing protocols for highly polyploid crops, including sugarcane (

Identifiants

pubmed: 34713257
doi: 10.3389/fgeed.2021.654996
pmc: PMC8525377
doi:

Types de publication

Journal Article

Langues

eng

Pagination

654996

Informations de copyright

Copyright © 2021 Eid, Mohan, Sanchez, Wang and Altpeter.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Ayman Eid (A)

Agronomy Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, United States.
Department of Energy Center for Advanced Bioenergy and Bioproducts Innovation, Gainesville, FL, United States.

Chakravarthi Mohan (C)

Department of Energy Center for Advanced Bioenergy and Bioproducts Innovation, Gainesville, FL, United States.

Sara Sanchez (S)

Agronomy Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, United States.
Department of Energy Center for Advanced Bioenergy and Bioproducts Innovation, Gainesville, FL, United States.

Duoduo Wang (D)

Agronomy Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, United States.
Department of Energy Center for Advanced Bioenergy and Bioproducts Innovation, Gainesville, FL, United States.

Fredy Altpeter (F)

Agronomy Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, United States.
Department of Energy Center for Advanced Bioenergy and Bioproducts Innovation, Gainesville, FL, United States.
Genetics Institute, University of Florida, Gainesville, FL, United States.
Plant Molecular and Cellular Biology Program, Institute of Food and Agricultural Sciences, Gainesville, FL, United States.

Classifications MeSH