Cryopreservation of dog epididymal spermatozoa by conventional freezing or ultra-rapid freezing with nonpermeable cryoprotectant.
Cryopreservation
Dog sperm
Epididymis
Slow freezing
Ultra-rapid freezing
Journal
Cryobiology
ISSN: 1090-2392
Titre abrégé: Cryobiology
Pays: Netherlands
ID NLM: 0006252
Informations de publication
Date de publication:
12 2021
12 2021
Historique:
received:
15
07
2021
revised:
20
10
2021
accepted:
24
10
2021
pubmed:
30
10
2021
medline:
29
1
2022
entrez:
29
10
2021
Statut:
ppublish
Résumé
This study was aimed to assess the effectiveness of two methods for cryopreservation of dog epididymal spermatozoa, one by conventional freezing (CF) with shortening both equilibration and cooling times, and the other by ultra-rapid freezing (URF) with nonpermeable cryoprotectant. Sixty epididymides were recovered from thirty orchiectomized adult dogs and the sperm samples were retrieved by retrograde flushing using TCG-EY (tris, citric acid, glucose + 20% egg yolk) extender and then 20 pools were conformed. Each pool was divided into 2 aliquots and then cryopreserved by CF and URF methods respectively. The CF method maintained the cooled-pool samples for 2h (1h without and 1h with 5% glycerol) and then were frozen by liquid nitrogen (LN
Identifiants
pubmed: 34715114
pii: S0011-2240(21)00174-7
doi: 10.1016/j.cryobiol.2021.10.002
pii:
doi:
Substances chimiques
Cryoprotective Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
15-21Informations de copyright
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