Developmental Timing of Trauma in Women Predicts Unique Extracellular Vesicle Proteome Signatures.
Biomarker
Development
Extracellular vesicle
Keratin
Proteomics
Trauma
Journal
Biological psychiatry
ISSN: 1873-2402
Titre abrégé: Biol Psychiatry
Pays: United States
ID NLM: 0213264
Informations de publication
Date de publication:
01 02 2022
01 02 2022
Historique:
received:
13
04
2021
revised:
05
08
2021
accepted:
05
08
2021
pubmed:
31
10
2021
medline:
29
1
2022
entrez:
30
10
2021
Statut:
ppublish
Résumé
Exposure to traumatic events is a risk factor for negative physical and mental health outcomes. However, the underlying biological mechanisms that perpetuate these lasting effects are not known. We investigated the impact and timing of sexual trauma, a specific type of interpersonal violence, experienced during key developmental windows of childhood, adolescence, or adulthood on adult health outcomes and associated biomarkers, including circulating cell-free mitochondrial DNA levels and extracellular vesicles (EVs), in a predominantly Black cohort of women (N = 101). Significant changes in both biomarkers examined, circulating cell-free mitochondrial DNA levels and EV proteome, were specific to developmental timing of sexual trauma. Specifically, we identified a large number of keratin-related proteins from EVs unique to the adolescent sexual trauma group. Remarkably, the majority of these keratin proteins belong to a 17q21 gene cluster, which suggests a potential local epigenetic regulatory mechanism altered by adolescent trauma to impact keratinocyte EV secretion or its protein cargo. These results, along with changes in fear-potentiated startle and skin conductance detected in these women, suggest that sexual violence experienced during the specific developmental window of adolescence may involve unique programming of the skin, the body's largest stress organ. Together, these descriptive studies provide novel insight into distinct biological processes altered by trauma experienced during specific developmental windows. Future studies will be required to mechanistically link these biological processes to health outcomes.
Sections du résumé
BACKGROUND
Exposure to traumatic events is a risk factor for negative physical and mental health outcomes. However, the underlying biological mechanisms that perpetuate these lasting effects are not known.
METHODS
We investigated the impact and timing of sexual trauma, a specific type of interpersonal violence, experienced during key developmental windows of childhood, adolescence, or adulthood on adult health outcomes and associated biomarkers, including circulating cell-free mitochondrial DNA levels and extracellular vesicles (EVs), in a predominantly Black cohort of women (N = 101).
RESULTS
Significant changes in both biomarkers examined, circulating cell-free mitochondrial DNA levels and EV proteome, were specific to developmental timing of sexual trauma. Specifically, we identified a large number of keratin-related proteins from EVs unique to the adolescent sexual trauma group. Remarkably, the majority of these keratin proteins belong to a 17q21 gene cluster, which suggests a potential local epigenetic regulatory mechanism altered by adolescent trauma to impact keratinocyte EV secretion or its protein cargo. These results, along with changes in fear-potentiated startle and skin conductance detected in these women, suggest that sexual violence experienced during the specific developmental window of adolescence may involve unique programming of the skin, the body's largest stress organ.
CONCLUSIONS
Together, these descriptive studies provide novel insight into distinct biological processes altered by trauma experienced during specific developmental windows. Future studies will be required to mechanistically link these biological processes to health outcomes.
Identifiants
pubmed: 34715991
pii: S0006-3223(21)01521-3
doi: 10.1016/j.biopsych.2021.08.003
pmc: PMC9219961
mid: NIHMS1751643
pii:
doi:
Substances chimiques
Proteome
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
273-282Subventions
Organisme : NICHD NIH HHS
ID : R01 HD097093
Pays : United States
Organisme : NIMH NIH HHS
ID : R33 MH104184
Pays : United States
Organisme : NIEHS NIH HHS
ID : R01 ES028202
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH111682
Pays : United States
Organisme : NIMH NIH HHS
ID : R37 MH108286
Pays : United States
Organisme : NICHD NIH HHS
ID : K99 HD091376
Pays : United States
Organisme : NIMH NIH HHS
ID : R21 MH098212
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH110364
Pays : United States
Organisme : NICHD NIH HHS
ID : R00 HD091376
Pays : United States
Organisme : NIMH NIH HHS
ID : R21 MH104184
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2021 Society of Biological Psychiatry. Published by Elsevier Inc. All rights reserved.
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