Dynamics of urinary and respiratory shedding of Severe acute respiratory syndrome virus 2 (SARS-CoV-2) RNA excludes urine as a relevant source of viral transmission.

COVID-19 / diagnosis Humans Male RNA, Viral Severe acute respiratory syndrome-related coronavirus SARS-CoV-2 / genetics Urinary Tract / chemistry Virus Shedding

COVID-19 Severe acute respiratory syndrome coronavirus 2 Urinary tract Viral shedding

Journal

Infection

ISSN: 1439-0973

Titre abrégé: Infection

Pays: Germany

ID NLM: 0365307

Informations de publication

Date de publication:
Jun 2022

Historique:

received: 31 05 2021

accepted: 17 09 2021

pubmed: 31 10 2021

medline: 3 6 2022

entrez: 30 10 2021

Statut: ppublish

Résumé

To investigate the expression of the receptor protein ACE-2 alongside the urinary tract, urinary shedding and urinary stability of SARS-CoV-2 RNA. Immunohistochemical staining was performed on tissue from urological surgery of 10 patients. Further, patients treated for coronavirus disease (COVID-19) at specialized care-units of a university hospital were assessed for detection of SARS-CoV-2 RNA in urinary samples via PCR, disease severity (WHO score), inflammatory response of patients. Finally, the stability of SARS-CoV-2 RNA in urine was analyzed. High ACE-2 expression (3/3) was observed in the tubules of the kidney and prostate glands, moderate expression in urothelial cells of the bladder (0-2/3) and no expression in kidney glomeruli, muscularis of the bladder and stroma of the prostate (0/3). SARS-CoV-2 RNA was detected in 5/199 urine samples from 64 patients. Viral RNA was detected in the first urinary sample of sequential samples. Viral RNA load from other specimen as nasopharyngeal swabs (NPS) or endotracheal aspirates revealed higher levels than from urine. Detection of SARS-CoV-2 RNA in urine was not associated with impaired WHO score (median 5, range 3-8 vs median 4, range 1-8, p = 0.314), peak white blood cell count (median 24.1 × 1000/ml, range 5.19-48.1 versus median 11.9 × 1000/ml, range 2.9-60.3, p = 0.307), peak CRP (median 20.7 mg/dl, 4.2-40.2 versus median 11.9 mg/dl, range 0.1-51.9, p = 0.316) or peak IL-6 levels (median: 1442 ng/ml, range 26.7-3918 versus median 140 ng/ml, range 3.0-11,041, p = 0.099). SARS-CoV-2 RNA was stable under different storage conditions and after freeze-thaw cycles. SARS-CoV-2 RNA in the urine of COVID-19 patients occurs infrequently. The viral RNA load and dynamics of SARS-CoV-2 RNA shedding suggest no relevant route of transmission through the urinary tract.

Identifiants

DOI: 10.1007/s15010-021-01724-4 PMID: 34716901 PMC: PMC8556791

pubmed: 34716901

doi: 10.1007/s15010-021-01724-4

pii: 10.1007/s15010-021-01724-4

pmc: PMC8556791

doi:

Substances chimiques

RNA, Viral 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

635-642

Subventions

Organisme : Medizinischen Fakultät, Ludwig-Maximilians-Universität München

ID : Cluster 2

Organisme : Medizinischen Fakultät, Ludwig-Maximilians-Universität München

ID : LMUexcellent

Organisme : Horst-Lühl-Foundation

ID : Inflammatory mechanisms in urothelial carcinoma

Organisme : Bundesministerium für Bildung und Forschung

ID : NaFoUniMedCovid19 (01KX2021)

Organisme : Bundesministerium für Bildung und Forschung

ID : B-Fast

Informations de copyright

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