Seasonal genotype dynamics of a marine dinoflagellate: Pelagic populations are homogeneous and as diverse as benthic seed banks.
Alexandrium ostenfeldii
adaptation
evolution
phytoplankton
resting stage
selection
Journal
Molecular ecology
ISSN: 1365-294X
Titre abrégé: Mol Ecol
Pays: England
ID NLM: 9214478
Informations de publication
Date de publication:
01 2022
01 2022
Historique:
revised:
19
10
2021
received:
27
08
2021
accepted:
25
10
2021
pubmed:
31
10
2021
medline:
29
1
2022
entrez:
30
10
2021
Statut:
ppublish
Résumé
Genetic diversity is the basis for evolutionary adaptation and selection under changing environmental conditions. Phytoplankton populations are genotypically diverse, can become genetically differentiated within small spatiotemporal scales and many species form resting stages. Resting stage accumulations in sediments (seed banks) are expected to serve as reservoirs for genetic information, but so far their role in maintaining phytoplankton diversity and in evolution has remained unclear. In this study we used the toxic dinoflagellate Alexandrium ostenfeldii (Dinophyceae) as a model organism to investigate if (i) the benthic seed bank is more diverse than the pelagic population and (ii) the pelagic population is seasonally differentiated. Resting stages (benthic) and plankton (pelagic) samples were collected at a coastal bloom site in the Baltic Sea, followed by cell isolation and genotyping using microsatellite markers (MS) and restriction site associated DNA sequencing (RAD). High clonal diversity (98%-100%) combined with intermediate to low gene diversity (0.58-0.03, depending on the marker) was found. Surprisingly, the benthic and pelagic fractions of the population were equally diverse, and the pelagic fraction was temporally homogeneous, despite seasonal fluctuation of environmental selection pressures. The results of this study suggest that continuous benthic-pelagic coupling, combined with frequent sexual reproduction, as indicated by persistent linkage equilibrium, prevent the dominance of single clonal lineages in a dynamic environment. Both processes harmonize the pelagic with the benthic population and thus prevent seasonal population differentiation. At the same time, frequent sexual reproduction and benthic-pelagic coupling maintain high clonal diversity in both habitats.
Identifiants
pubmed: 34716943
doi: 10.1111/mec.16257
pmc: PMC9298838
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
512-528Subventions
Organisme : Academy of Finland
ID : 310449
Organisme : Academy of Finland
ID : 251564
Organisme : Walter ja Andrée de Nottbeckin Säätiö
Organisme : Japan Society for the Promotion of Science
ID : 18KK0182
Informations de copyright
© 2021 The Authors. Molecular Ecology published by John Wiley & Sons Ltd.
Références
Science. 2014 Apr 25;344(6182):416-20
pubmed: 24763590
Mol Ecol Resour. 2012 Nov;12(6):1158-60
pubmed: 22883857
PLoS Biol. 2010 Apr 27;8(4):e1000357
pubmed: 20463950
ISME J. 2018 Feb;12(2):463-472
pubmed: 29160864
Mol Ecol. 2010 Oct;19(20):4478-90
pubmed: 20875064
Bioinformatics. 2011 Nov 1;27(21):3070-1
pubmed: 21926124
Ecol Evol. 2012 Oct;2(10):2588-99
pubmed: 23145343
Mar Genomics. 2012 Sep;7:51-6
pubmed: 22897963
Mol Ecol. 2022 Jan;31(2):512-528
pubmed: 34716943
Genetics. 1980 Oct;96(2):523-36
pubmed: 17249067
BMC Evol Biol. 2010 Jan 13;10:11
pubmed: 20070898
Mol Ecol. 2015 Jun;24(12):2955-72
pubmed: 25919789
Environ Microbiol. 2018 Aug;20(8):2783-2795
pubmed: 29614214
Mol Ecol. 2014 Feb;23(3):549-60
pubmed: 24330231
Mol Ecol. 2005 May;14(6):1631-40
pubmed: 15836638
Biotechniques. 1999 Jul;27(1):66-8
pubmed: 10407666
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3321-3
pubmed: 4519626
Harmful Algae. 2017 Mar;63:146-153
pubmed: 28366389
G3 (Bethesda). 2011 Aug;1(3):171-82
pubmed: 22384329
Genetics. 1978 Jul;89(3):583-90
pubmed: 17248844
PLoS One. 2011;6(7):e22965
pubmed: 21829565
Evolution. 2013 Jul;67(7):1849-59
pubmed: 23815643
Nat Protoc. 2017 Dec;12(12):2640-2659
pubmed: 29189774
Mol Ecol. 2013 Jun;22(11):3179-90
pubmed: 23551379
J Phycol. 2012 Oct;48(5):1197-208
pubmed: 27011279
Proc Natl Acad Sci U S A. 2008 Apr 1;105(13):5134-8
pubmed: 18375765
Mol Ecol. 2021 Feb;30(4):912-925
pubmed: 33386639
Ecol Lett. 2009 Aug;12(8):744-57
pubmed: 19580586
Environ Microbiol. 2012 Sep;14(9):2395-404
pubmed: 22568551
Glob Chang Biol. 2019 Sep;25(9):2869-2884
pubmed: 31058393
Environ Microbiol. 2016 Feb;18(2):679-91
pubmed: 26913820
Nat Ecol Evol. 2018 Apr;2(4):611-613
pubmed: 29434348
PeerJ. 2014 Mar 04;2:e281
pubmed: 24688859
J Phycol. 2019 Dec;55(6):1226-1238
pubmed: 31520419
Mol Ecol. 2013 Jun;22(11):3124-40
pubmed: 23701397
Genetics. 2011 Aug;188(4):799-808
pubmed: 21828280
Evol Appl. 2014 Jan;7(1):140-55
pubmed: 24454553
Harmful Algae. 2019 Apr;84:64-74
pubmed: 31128814
Science. 1978 Mar 24;199(4335):1302-10
pubmed: 17840770
Science. 1998 Jul 10;281(5374):237-40
pubmed: 9657713
Harmful Algae. 2019 Apr;84:181-187
pubmed: 31128802
Nat Commun. 2011;2:311
pubmed: 21587228
ISME J. 2016 Nov;10(11):2755-2766
pubmed: 27046335
PLoS One. 2012;7(12):e53602
pubmed: 23300940
Mol Ecol. 2020 Dec;29(24):4913-4924
pubmed: 32672394
Mol Ecol. 2009 May;18(10):2122-33
pubmed: 19389181
PLoS One. 2011;6(12):e28012
pubmed: 22164224
J Eukaryot Microbiol. 2017 Mar;64(2):248-256
pubmed: 27543207
Harmful Algae. 2019 Jun;86:46-54
pubmed: 31358276
Front Genet. 2015 Jun 10;6:208
pubmed: 26113860
Mol Ecol. 2015 Jun;24(11):2871-85
pubmed: 25892181
PLoS One. 2011 Apr 13;6(4):e18561
pubmed: 21541009
ISME J. 2018 Dec;12(12):2929-2941
pubmed: 30068937
Proc Natl Acad Sci U S A. 2011 Mar 8;108(10):4252-7
pubmed: 21282612
Genome Biol. 2019 Nov 28;20(1):257
pubmed: 31779668
Philos Trans R Soc Lond B Biol Sci. 2017 Sep 5;372(1728):
pubmed: 28717025
Environ Microbiol. 2015 Dec;17(12):5063-72
pubmed: 26184488
Ecol Evol. 2019 Apr 01;9(8):4443-4451
pubmed: 31031918
BMC Genomics. 2017 Jan 11;18(1):69
pubmed: 28077077
Sci Rep. 2017 Dec 14;7(1):17598
pubmed: 29242627