Effects of the donor factors and freezing protocols on the bovine embryonic lipid profile†.
N-3 PUFA
bovine
cryopreservation
embryo quality
in vitro embryo production
lipidomic
Journal
Biology of reproduction
ISSN: 1529-7268
Titre abrégé: Biol Reprod
Pays: United States
ID NLM: 0207224
Informations de publication
Date de publication:
19 03 2022
19 03 2022
Historique:
received:
21
07
2021
revised:
24
09
2021
accepted:
25
10
2021
pubmed:
1
11
2021
medline:
3
5
2022
entrez:
31
10
2021
Statut:
ppublish
Résumé
Embryo lipid profile is affected by in vitro culture conditions that lead to an increase in lipids. Efforts have been made to optimize embryo lipid composition as it is associated with their quality. The objective of this study was to evaluate whether the diet supplementation of donor cows (n-3 or n-6 polyunsaturated fatty acids), or the slow freezing protocols (ethylene glycol sucrose vs. glycerol-trehalose), or the physiological stage of the donor (nulliparous heifers vs. primiparous lactating cows) may impact the bovine embryo lipid profile. Lipid extracts of 97 embryos were individually analyzed by liquid chromatography-high resolution mass spectrometry, highlighting 246 lipids, including 85% being overabundant in cow embryos compared to heifer embryos. Among 105 differential lipids, 72 were overabundant after ethylene glycol sucrose protocol, including a single glycerophosphate PA(32:1) representing 27.3% of the significantly modulated lipids, suggesting that it is degraded when glycerol-trehalose protocol is used. No lipids were different according to the n-3 or n-6 supplementation of the donor cows. In conclusion, the embryonic lipid profile was mainly affected by the physiological stage of the donors and the slow freezing protocols. The overabundance of lipids in lactating cow embryos and the resulting lower quality of these embryos are consistent with the lower pregnancy rate observed in cows compared to heifers. Unlike glycerol-trehalose protocol, ethylene glycol sucrose freezing allowed to preserve glycerophospholipids, potentially improving the slow freezing of in vitro-produced embryos. Further studies are required to modulate embryo quality and freezability by modulating the lipidome and by integrating all stages of embryonic production.
Identifiants
pubmed: 34718415
pii: 6414251
doi: 10.1093/biolre/ioab198
pmc: PMC8934692
doi:
Substances chimiques
Ethylene Glycols
0
Lipids
0
Sucrose
57-50-1
Trehalose
B8WCK70T7I
Glycerol
PDC6A3C0OX
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
597-612Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
Références
Nat Protoc. 2013 Jan;8(1):17-32
pubmed: 23222455
Biotechnol Prog. 2017 Jan;33(1):229-235
pubmed: 27802564
Comp Biochem Physiol A Mol Integr Physiol. 2002 Mar;131(3):515-25
pubmed: 11867277
Nat Rev Mol Cell Biol. 2003 May;4(5):414-8
pubmed: 12728275
Theriogenology. 2000 Jan 15;53(2):575-97
pubmed: 10735051
Theriogenology. 2006 Sep 15;66(5):1316-24
pubmed: 16735061
J Lipid Res. 2009 Apr;50 Suppl:S9-14
pubmed: 19098281
Cryobiology. 2021 Apr;99:140-148
pubmed: 33242477
Theriogenology. 2016 Jun;85(9):1625-1634.e2
pubmed: 26898414
Biol Reprod. 2017 Jan 1;97(6):892-901
pubmed: 29121172
Reprod Domest Anim. 2020 Jun;55(6):659-676
pubmed: 32144939
J Reprod Fertil. 1996 Mar;106(2):299-306
pubmed: 8699414
Biology (Basel). 2014 Nov 19;3(4):801-30
pubmed: 25415055
J Ovarian Res. 2017 Nov 9;10(1):74
pubmed: 29122003
Reprod Fertil Dev. 2016 Oct;28(12):1882-1892
pubmed: 26072962
J Dairy Sci. 2001 Jun;84(6):1277-93
pubmed: 11417685
J Dairy Sci. 2004 Apr;87(4):921-32
pubmed: 15259226
Biochim Biophys Acta. 2016 Jun;1858(6):1400-9
pubmed: 27003129
Hum Reprod. 2000 Oct;15(10):2160-4
pubmed: 11006192
Reprod Biomed Online. 2007 Apr;14(4):464-70
pubmed: 17425829
J Anim Sci. 2013 Feb;91(2):713-21
pubmed: 23148256
Anim Reprod Sci. 2006 Oct;95(3-4):193-205
pubmed: 16236474
Theriogenology. 1996 Feb;45(3):573-81
pubmed: 16727820
Theriogenology. 2009 Apr 1;71(6):947-58
pubmed: 19117603
Reprod Nutr Dev. 2004 Nov-Dec;44(6):551-64
pubmed: 15762299
Theriogenology. 2016 Jul 1;86(1):270-7
pubmed: 27158131
J Lipid Res. 2005 May;46(5):839-61
pubmed: 15722563
Theriogenology. 2019 Feb;125:277-284
pubmed: 30497026
Nat Rev Mol Cell Biol. 2018 May;19(5):281-296
pubmed: 29410529
Theriogenology. 2011 Apr 15;75(7):1211-20
pubmed: 21247620
Biol Reprod. 2009 Jan;80(1):70-8
pubmed: 18815355
Cryobiology. 2003 Jun;46(3):205-29
pubmed: 12818211
Biol Reprod. 1988 Jun;38(5):1171-80
pubmed: 3408784
Hum Reprod. 2005 Dec;20(12):3385-9
pubmed: 16055458
J Assist Reprod Genet. 2013 Aug;30(8):1091-8
pubmed: 23888311
J Dairy Sci. 2002 Nov;85(11):2813-22
pubmed: 12487448
Anim Reprod Sci. 2001 Jan 31;65(1-2):17-31
pubmed: 11182505
Biol Reprod. 2012 Dec 06;87(6):130
pubmed: 23053436
Reproduction. 2019 Jul;158(1):71-83
pubmed: 31013477
Reproduction. 2013 Oct 26;146(6):603-14
pubmed: 24062566
J Anim Sci. 2004;82 E-Suppl:E63-74
pubmed: 15471816
Biopreserv Biobank. 2019;17(1):76-83
pubmed: 30256133
Theriogenology. 2002 Jan 1;57(1):345-59
pubmed: 11775979
J Reprod Fertil. 1999 Jul;116(2):373-8
pubmed: 10615263
Reprod Fertil Dev. 2017 Jun;29(6):1217-1230
pubmed: 27220988
Reprod Fertil Dev. 2010;22(1):119-25
pubmed: 20003853
Theriogenology. 1997 Sep;48(4):563-79
pubmed: 16728153
Sci Rep. 2021 Jun 2;11(1):11618
pubmed: 34078963
Reprod Biol Endocrinol. 2015 May 16;13:44
pubmed: 25981539
Physiol Genomics. 2014 Jan 15;46(2):39-56
pubmed: 24220328
Exp Cell Res. 1972 Jan;70(1):41-8
pubmed: 5008405
Theriogenology. 2009 Jan 15;71(2):355-68
pubmed: 18801561
Anim Reprod Sci. 2008 Jul;106(3-4):322-32
pubmed: 17580103
Domest Anim Endocrinol. 2016 Jan;54:15-29
pubmed: 26379100
J Reprod Fertil. 1998 Jan;112(1):123-30
pubmed: 9538337
J Reprod Dev. 2019 Oct 23;65(5):389-396
pubmed: 31189772
Biochim Biophys Acta. 1988 Dec 22;946(2):193-201
pubmed: 3207735
J Reprod Dev. 2003 Jun;49(3):193-202
pubmed: 14967928
Theriogenology. 2005 Dec;64(9):2022-36
pubmed: 15936067
J Dairy Sci. 2018 Oct;101(10):8641-8661
pubmed: 30100509
J Nutr. 2012 Mar;142(3):592S-599S
pubmed: 22279140
J Dairy Sci. 2016 Dec;99(12):10109-10127
pubmed: 27692709
Cryobiology. 1999 Mar;38(2):95-105
pubmed: 10191033
Cryobiology. 1996 Dec;33(6):589-99
pubmed: 8975686