Phospholipase D and retromer promote recycling of TRPL ion channel via the endoplasmic reticulum.
Animals
Drosophila
/ metabolism
Drosophila Proteins
/ metabolism
Drosophila melanogaster
/ metabolism
Endoplasmic Reticulum
/ metabolism
Light
Phospholipase D
/ metabolism
Photoreceptor Cells, Invertebrate
/ metabolism
Protein Transport
/ physiology
Transient Receptor Potential Channels
/ metabolism
Drosophila photoreceptors
TRPL ion channel
endoplasmic reticulum
endosomal trafficking
membrane protein trafficking
neurodegeneration
phospholipase D
retinal degeneration
retromer complex
Journal
Traffic (Copenhagen, Denmark)
ISSN: 1600-0854
Titre abrégé: Traffic
Pays: England
ID NLM: 100939340
Informations de publication
Date de publication:
01 2022
01 2022
Historique:
revised:
20
10
2021
received:
25
03
2021
accepted:
26
10
2021
pubmed:
1
11
2021
medline:
4
5
2022
entrez:
31
10
2021
Statut:
ppublish
Résumé
Plasma membrane protein trafficking is of fundamental importance for cell function and cell integrity of neurons and includes regulated protein recycling. In this work, we report a novel role of the endoplasmic reticulum (ER) for protein recycling as discovered in trafficking studies of the ion channel TRPL in photoreceptor cells of Drosophila. TRPL is located within the rhabdomeric membrane from where it is endocytosed upon light stimulation and stored in the cell body. Conventional immunohistochemistry as well as stimulated emission depletion super-resolution microscopy revealed TRPL storage at the ER after illumination, suggesting an unusual recycling route of TRPL. Our results also imply that both phospholipase D (PLD) and retromer complex are required for correct recycling of TRPL to the rhabdomeric membrane. Loss of PLD activity in PLD
Substances chimiques
Drosophila Proteins
0
Transient Receptor Potential Channels
0
Phospholipase D
EC 3.1.4.4
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
42-62Informations de copyright
© 2021 The Authors. Traffic published by John Wiley & Sons Ltd.
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