Comparison of Molecular and Parasitological Methods for Diagnosis of Human Trichostrongylosis.

Harada–Mori culture PCR Willis agar plate culture formalin ethyl acetate concentration human trichostrongyliasis wet mount

Journal

Frontiers in cellular and infection microbiology
ISSN: 2235-2988
Titre abrégé: Front Cell Infect Microbiol
Pays: Switzerland
ID NLM: 101585359

Informations de publication

Date de publication:
2021
Historique:
received: 16 08 2021
accepted: 20 09 2021
entrez: 1 11 2021
pubmed: 2 11 2021
medline: 26 11 2021
Statut: epublish

Résumé

Human trichostrongyliasis is a zoonotic disease that is prevalent among rural populations in some countries. This study was performed to evaluate various parasitological methods and polymerase chain reaction (PCR) for the diagnosis of human trichostrongyliasis. A total of 206 fresh stool samples were collected from residents of endemic villages of Northern Iran. All samples were examined using conventional parasitological methods, including wet mount, formalin ethyl acetate concentration (FEAC), agar plate culture (APC), Harada-Mori culture (HMC), and Willis, along with the PCR technique. Among the total of 206 individuals examined, 72 people (35%) were found infected with

Identifiants

pubmed: 34722344
doi: 10.3389/fcimb.2021.759396
pmc: PMC8548760
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

759396

Informations de copyright

Copyright © 2021 Pandi, Sharifdini, Ashrafi, Atrkar Roushan, Rahmati and Hajipour.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Mehdi Pandi (M)

Department of Medical Parasitology and Mycology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.

Meysam Sharifdini (M)

Department of Medical Parasitology and Mycology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.

Keyhan Ashrafi (K)

Department of Medical Parasitology and Mycology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.

Zahra Atrkar Roushan (Z)

Department of Biostatistics, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.

Behnaz Rahmati (B)

Department of Medical Parasitology and Mycology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.

Nayereh Hajipour (N)

Department of Microbiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.

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Classifications MeSH