Murine Embryonic Stem Cell Culture, Self-Renewal, and Differentiation.
Cell culture
Differentiation
Knockout serum replacement
Leukemia inhibitory factor
Murine embryonic stem cells
Self-renewal
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
pubmed:
2
11
2021
medline:
28
7
2022
entrez:
1
11
2021
Statut:
ppublish
Résumé
Embryonic stem cells (ESCs), derived from the inner cell mass of the blastocyst, can proliferate indefinitely in vitro (self-renewal) and can differentiate into cells of all three germ layers (pluripotency). These unique properties make them exceptionally valuable in basic science and clinical researches, including cell replacement therapies, drug discovery, and regenerative medicine. Mouse ESCs represent an important model system for studying gene function during development and disease.ESCs culture is time-consuming, laborious, and costly. Suboptimal ESCs culture conditions can alter their identity, pluripotency, and their compatibility with downstream differentiation protocols. In this chapter, we provide a general guideline for murine ESCs culture on murine fibroblast feeder layers. Moreover, we describe protocols for maintenance of ESCs pluripotency and induction of ESCs differentiation.
Identifiants
pubmed: 34724189
doi: 10.1007/7651_2021_447
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
265-273Informations de copyright
© 2021. Springer Science+Business Media, LLC.
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