Induced pluripotency in the context of stem cell expansion bioprocess development, optimization, and manufacturing: a roadmap to the clinic.


Journal

NPJ Regenerative medicine
ISSN: 2057-3995
Titre abrégé: NPJ Regen Med
Pays: United States
ID NLM: 101699846

Informations de publication

Date de publication:
01 Nov 2021
Historique:
received: 13 05 2021
accepted: 07 10 2021
entrez: 2 11 2021
pubmed: 3 11 2021
medline: 3 11 2021
Statut: epublish

Résumé

The translation of laboratory-scale bioprocess protocols and technologies to industrial scales and the application of human induced pluripotent stem cell (hiPSC) derivatives in clinical trials globally presents optimism for the future of stem-cell products to impact healthcare. However, while many promising therapeutic approaches are being tested in pre-clinical studies, hiPSC-derived products currently account for a small fraction of active clinical trials. The complexity and volatility of hiPSCs present several bioprocessing challenges, where the goal is to generate a sufficiently large, high-quality, homogeneous population for downstream differentiation-the derivatives of which must retain functional efficacy and meet regulatory safety criteria in application. It is argued herein that one of the major challenges currently faced in improving the robustness of routine stem-cell biomanufacturing is in utilizing continuous, meaningful assessments of molecular and cellular characteristics from process to application. This includes integrating process data with biological characteristic and functional assessment data to model the interplay between variables in the search for global optimization strategies. Coupling complete datasets with relevant computational methods will contribute significantly to model development and automation in achieving process robustness. This overarching approach is thus crucially important in realizing the potential of hiPSC biomanufacturing for transformation of regenerative medicine and the healthcare industry.

Identifiants

pubmed: 34725374
doi: 10.1038/s41536-021-00183-7
pii: 10.1038/s41536-021-00183-7
pmc: PMC8560749
doi:

Types de publication

Journal Article Review

Langues

eng

Pagination

72

Informations de copyright

© 2021. The Author(s).

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Auteurs

James Colter (J)

Pharmaceutical Production Research Facility, University of Calgary, 2500 University Drive NW, Calgary, AB, T2N 1N4, Canada.
Integrated Circuits and Optical Imaging Laboratory, University of Calgary, 2500 University Drive NW, Calgary, AB, T2N 1N4, Canada.
Biomedical Engineering Graduate Program, University of Calgary, 2500 University Drive NW, Calgary, AB, T2N 1N4, Canada.

Kartikeya Murari (K)

Integrated Circuits and Optical Imaging Laboratory, University of Calgary, 2500 University Drive NW, Calgary, AB, T2N 1N4, Canada.
Biomedical Engineering Graduate Program, University of Calgary, 2500 University Drive NW, Calgary, AB, T2N 1N4, Canada.
Department of Electrical and Software Engineering, Schulich School of Engineering, University of Calgary, 2500 University Drive NW, Calgary, AB, T2N 1N4, Canada.

Jeff Biernaskie (J)

Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, 2500 University Drive NW, Calgary, AB, T2N 1N4, Canada.
Department of Surgery,Cumming School of Medicine, University of Calgary, Calgar, AB, Canada.
Hotchkiss Brain Institute, University of Calgary, Calgary, AB, Canada.
Alberta Children's Hospital Research Institute, University of Calgary, Calgary, AB, Canada.

Michael Scott Kallos (MS)

Pharmaceutical Production Research Facility, University of Calgary, 2500 University Drive NW, Calgary, AB, T2N 1N4, Canada. mskallos@ucalgary.ca.
Biomedical Engineering Graduate Program, University of Calgary, 2500 University Drive NW, Calgary, AB, T2N 1N4, Canada. mskallos@ucalgary.ca.
Department of Chemical and Petroleum Engineering, Schulich School of Engineering, University of Calgary, 2500 University Drive NW, Calgary, AB, T2N 1N4, Canada. mskallos@ucalgary.ca.

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