Enhanced cytotoxic activity of beta carotene conjugated liposomes towards breast cancer cell line: comparative studies with cyclophosphamide.
Antineoplastic Agents, Alkylating
/ administration & dosage
Breast Neoplasms
/ pathology
Cyclophosphamide
/ administration & dosage
DNA Damage
/ drug effects
Dose-Response Relationship, Drug
Drug Carriers
/ chemistry
Drug Combinations
Drug Liberation
Drug Stability
Female
Humans
Liposomes
/ chemistry
MCF-7 Cells
Particle Size
Surface Properties
Temperature
beta Carotene
/ administration & dosage
Journal
Anti-cancer drugs
ISSN: 1473-5741
Titre abrégé: Anticancer Drugs
Pays: England
ID NLM: 9100823
Informations de publication
Date de publication:
01 01 2022
01 01 2022
Historique:
pubmed:
3
11
2021
medline:
5
3
2022
entrez:
2
11
2021
Statut:
ppublish
Résumé
This work aims to evaluate cyclophosphamide (Cyclo) cytotoxic efficacy combined with liposomes in the presence or absence of beta carotene (beta) by detecting the effects of these compounds on the breast cancer cell line (MCF-7) DNA damage. The IC50 value for beta in cytotoxic assay with MCF-7 treated cells was 21.15 μg/ml, while with liposomal beta (LipoBeta) being 121 μg/ml. The free Cyclo IC50 value was 719.86 μg/ml, its liposomal form (LipoCyclo) was 172 μg/ml. The results indicated that in contrast with Cyclo and control values, all comet assay parameters for the LipoBeta were significantly increased (P < 0.05). In MCF-7 cells treated with beta, the findings show a higher intensity of comet tail than those treated with LipoBeta. The presence of several double-strand breaks suggests this high intensity relative to the head. The molecular combination between Cyclo and liposomes in the presence or absence of beta was characterized. Dynamic light scattering measurements confirmed the mono-dispersity of all samples. The incorporation of Cyclo or beta into liposomes exhibited a slight shift to higher temperature compared to the main peak of empty liposomes that exists at 101.5°C which creates a conformational disorder within the phospholipids. The FTIR study showed structural alterations in vesicles after liposome encapsulation.
Identifiants
pubmed: 34726638
doi: 10.1097/CAD.0000000000001245
pii: 00001813-202201000-00061
doi:
Substances chimiques
Antineoplastic Agents, Alkylating
0
Drug Carriers
0
Drug Combinations
0
Liposomes
0
beta Carotene
01YAE03M7J
Cyclophosphamide
8N3DW7272P
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e462-e476Informations de copyright
Copyright © 2021 Wolters Kluwer Health, Inc. All rights reserved.
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