Onchocerca volvulus transmission in the Mbam valley of Cameroon following 16 years of annual community-directed treatment with ivermectin, and the description of a new cytotype of Simulium squamosum.


Journal

Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774

Informations de publication

Date de publication:
02 Nov 2021
Historique:
received: 27 08 2021
accepted: 16 10 2021
entrez: 3 11 2021
pubmed: 4 11 2021
medline: 1 1 2022
Statut: epublish

Résumé

The onchocerciasis focus surrounding the lower Mbam and Sanaga rivers, where Onchocerca volvulus is transmitted by Simulium damnosum s.l. (Diptera: Simuliidae), was historically the largest in the southern regions of Cameroon. Annual community-directed treatment with ivermectin (CDTI) has been taking place since 2000, but recent studies have shown that new infections are occurring in children. We aimed to investigate blackfly biting and O. volvulus transmission rates along the lower Mbam river 16 years after the formal onset of annual CDTI. Black flies were collected for three consecutive days each month between July 2016 and June 2017 at two riverside villages and two inland sites situated 4.9 km and 7.9 km from the riverside. Specimens collected at each site were dissected on one of the three collection days each month to estimate parity rates and O. volvulus infection rates, while the remaining samples were preserved for pool screening. In total, 93,573 S. damnosum s.l. black flies were recorded biting humans and 9281 were dissected. Annual biting rates of up to 606,370 were estimated at the riverside, decreasing to 20,540 at 7.9 km, while, based on dissections, annual transmission potentials of up to 4488 were estimated at the riverside, decreasing to 102 and 0 at 4.9 km and 7.9 km, respectively. However, pool screening showed evidence of infection in black flies at the furthest distance from the river. Results of both methods demonstrated the percentage of infective flies to be relatively low (0.10-0.36%), but above the WHO threshold for interruption of transmission. In addition, a small number of larvae collected during the dry season revealed the presence of Simulium squamosum E. This is the first time S. squamosum E has been found east of Lake Volta in Ghana, but our material was chromosomally distinctive, and we call it S. squamosum E2. Relatively low O. volvulus infection rates appear to be offset by extremely high densities of biting black flies which are sustaining transmission along the banks of the lower Mbam river.

Sections du résumé

BACKGROUND BACKGROUND
The onchocerciasis focus surrounding the lower Mbam and Sanaga rivers, where Onchocerca volvulus is transmitted by Simulium damnosum s.l. (Diptera: Simuliidae), was historically the largest in the southern regions of Cameroon. Annual community-directed treatment with ivermectin (CDTI) has been taking place since 2000, but recent studies have shown that new infections are occurring in children. We aimed to investigate blackfly biting and O. volvulus transmission rates along the lower Mbam river 16 years after the formal onset of annual CDTI.
METHODS METHODS
Black flies were collected for three consecutive days each month between July 2016 and June 2017 at two riverside villages and two inland sites situated 4.9 km and 7.9 km from the riverside. Specimens collected at each site were dissected on one of the three collection days each month to estimate parity rates and O. volvulus infection rates, while the remaining samples were preserved for pool screening.
RESULTS RESULTS
In total, 93,573 S. damnosum s.l. black flies were recorded biting humans and 9281 were dissected. Annual biting rates of up to 606,370 were estimated at the riverside, decreasing to 20,540 at 7.9 km, while, based on dissections, annual transmission potentials of up to 4488 were estimated at the riverside, decreasing to 102 and 0 at 4.9 km and 7.9 km, respectively. However, pool screening showed evidence of infection in black flies at the furthest distance from the river. Results of both methods demonstrated the percentage of infective flies to be relatively low (0.10-0.36%), but above the WHO threshold for interruption of transmission. In addition, a small number of larvae collected during the dry season revealed the presence of Simulium squamosum E. This is the first time S. squamosum E has been found east of Lake Volta in Ghana, but our material was chromosomally distinctive, and we call it S. squamosum E2.
CONCLUSIONS CONCLUSIONS
Relatively low O. volvulus infection rates appear to be offset by extremely high densities of biting black flies which are sustaining transmission along the banks of the lower Mbam river.

Identifiants

pubmed: 34727965
doi: 10.1186/s13071-021-05072-y
pii: 10.1186/s13071-021-05072-y
pmc: PMC8561987
doi:

Substances chimiques

Insecticides 0
Ivermectin 70288-86-7

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

563

Subventions

Organisme : Departement Economie, Wetenschap en Innovatie
ID : 2014 SOFI 12
Organisme : H2020 European Research Council
ID : 671055
Organisme : Deutsches Zentrum für Infektionsforschung
ID : TI 03.907

Informations de copyright

© 2021. The Author(s).

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Auteurs

Adam Hendy (A)

Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium. adhendy@utmb.edu.
Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA. adhendy@utmb.edu.

Meryam Krit (M)

Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium.

Kenneth Pfarr (K)

Institute for Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Bonn, Germany.
German Center for Infection Research, Partner Site Bonn-Cologne, Cologne, Germany.

Christine Laemmer (C)

Institute for Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, Bonn, Germany.
German Center for Infection Research, Partner Site Bonn-Cologne, Cologne, Germany.

Jacobus De Witte (J)

Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium.

Philippe Nwane (P)

Centre for Research on Filariasis and Other Tropical Diseases (CRFilMT), Yaoundé, Cameroon.

Joseph Kamgno (J)

Centre for Research on Filariasis and Other Tropical Diseases (CRFilMT), Yaoundé, Cameroon.
Department of Public Health, Faculty of Medicine and Biomedical Sciences, The University of Yaoundé I, Yaoundé, Cameroon.

Hugues C Nana-Djeunga (HC)

Centre for Research on Filariasis and Other Tropical Diseases (CRFilMT), Yaoundé, Cameroon.

Michel Boussinesq (M)

Institut de Recherche pour le Développement (IRD), Montpellier, France.

Jean-Claude Dujardin (JC)

Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium.

Rory Post (R)

Department of Disease Control, London School of Hygiene and Tropical Medicine, London, UK.
School of Natural Sciences and Psychology, Liverpool John Moores University, Liverpool, UK.

Robert Colebunders (R)

Global Health Institute, University of Antwerp, Antwerp, Belgium.

Sarah O'Neill (S)

Department of Public Health, Institute of Tropical Medicine, Antwerp, Belgium.
CR 5, Ecole de Santé Publique, Université Libre de Bruxelles, Brussels, Belgium.

Peter Enyong (P)

Research Foundation in Tropical Diseases and Environment, Buea, Cameroon.

Alfred K Njamnshi (AK)

Neuroscience Lab, Faculty of Medicine and Biomedical Sciences, The University of Yaoundé I, Yaoundé, Cameroon.
Brain Research Africa Initiative (BRAIN), Yaoundé, Cameroon.
Brain Research Africa Initiative (BRAIN), Geneva, Switzerland.
Neurology Department, Central Hospital Yaoundé, Yaoundé, Cameroon.

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