Co-delivery of doxorubicin and conferone by novel pH-responsive β-cyclodextrin grafted micelles triggers apoptosis of metastatic human breast cancer cells.
Antibiotics, Antineoplastic
/ administration & dosage
Apoptosis
Breast Neoplasms
/ drug therapy
Cell Cycle
Cell Proliferation
Coumarins
/ administration & dosage
Doxorubicin
/ administration & dosage
Drug Delivery Systems
Female
Humans
Hydrogen-Ion Concentration
Micelles
Tumor Cells, Cultured
beta-Cyclodextrins
/ chemistry
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
02 11 2021
02 11 2021
Historique:
received:
11
07
2021
accepted:
05
10
2021
entrez:
3
11
2021
pubmed:
4
11
2021
medline:
27
1
2022
Statut:
epublish
Résumé
Adjuvant-aided combination chemotherapy is one of the most effective ways of cancer treatment by overcoming the multidrug resistance (MDR) and reducing the side-effects of anticancer drugs. In this study, Conferone (Conf) was used as an adjuvant in combination with Doxorubicin (Dox) for inducing apoptosis to MDA-MB-231 cells. Herein, the novel biodegradable amphiphilic β-cyclodextrin grafted poly maleate-co-PLGA was synthesized by thiol-ene addition and ring-opening process. Micelles obtained from the novel copolymer showed exceptional properties such as small size of around 34.5 nm, CMC of 0.1 μg/mL, and cell internalization of around 100% at 30 min. These novel engineered micelles were used for combination delivery of doxorubicin-conferone with high encapsulation efficiency of near 100% for both drugs. Our results show that combination delivery of Dox and Conf to MDA-MB-231 cells had synergistic effects (CI < 1). According to cell cycle and Annexin-V apoptosis analysis, Dox-Conf loaded micelle significantly induce tumor cell apoptosis (more than 98% of cells population showed apoptosis at IC
Identifiants
pubmed: 34728703
doi: 10.1038/s41598-021-00954-8
pii: 10.1038/s41598-021-00954-8
pmc: PMC8563731
doi:
Substances chimiques
Antibiotics, Antineoplastic
0
Coumarins
0
Micelles
0
beta-Cyclodextrins
0
conferone
0
Doxorubicin
80168379AG
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
21425Informations de copyright
© 2021. The Author(s).
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