Using soft X-ray tomography for rapid whole-cell quantitative imaging of SARS-CoV-2-infected cells.
SARS-CoV-2
cells
cryo-tomography
organelles
soft X-ray
synchrotron
tomography
viral replication organelles
Journal
Cell reports methods
ISSN: 2667-2375
Titre abrégé: Cell Rep Methods
Pays: United States
ID NLM: 9918227360606676
Informations de publication
Date de publication:
22 11 2021
22 11 2021
Historique:
received:
27
05
2021
revised:
10
09
2021
accepted:
22
10
2021
pubmed:
4
11
2021
medline:
4
11
2021
entrez:
3
11
2021
Statut:
ppublish
Résumé
High-resolution and rapid imaging of host cell ultrastructure can generate insights toward viral disease mechanism, for example for a severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. Here, we employ full-rotation soft X-ray tomography (SXT) to examine organelle remodeling induced by SARS-CoV-2 at the whole-cell level with high spatial resolution and throughput. Most of the current SXT systems suffer from a restricted field of view due to use of flat sample supports and artifacts due to missing data. In this approach using cylindrical sample holders, a full-rotation tomogram of human lung epithelial cells is performed in less than 10 min. We demonstrate the potential of SXT imaging by visualizing aggregates of SARS-CoV-2 virions and virus-induced intracellular alterations. This rapid whole-cell imaging approach allows us to visualize the spatiotemporal changes of cellular organelles upon viral infection in a quantitative manner.
Identifiants
pubmed: 34729550
doi: 10.1016/j.crmeth.2021.100117
pii: S2667-2375(21)00181-8
pmc: PMC8552653
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
100117Subventions
Organisme : NIGMS NIH HHS
ID : P30 GM138441
Pays : United States
Organisme : NIGMS NIH HHS
ID : P41 GM103445
Pays : United States
Informations de copyright
© 2021 The Authors.
Déclaration de conflit d'intérêts
The authors declare no competing interests.
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