Identification of two ABCC transporters involved in malathion detoxification in the red flour beetle, Tribolium castaneum.
ATP-binding cassette transporters (ABC transporter)
Tribolium castaneum
insecticide detoxification
malathion
tebufenozide
β-cyfluthrin
Journal
Insect science
ISSN: 1744-7917
Titre abrégé: Insect Sci
Pays: Australia
ID NLM: 101266965
Informations de publication
Date de publication:
Aug 2022
Aug 2022
Historique:
revised:
19
09
2021
received:
09
08
2021
accepted:
25
10
2021
pubmed:
4
11
2021
medline:
22
7
2022
entrez:
3
11
2021
Statut:
ppublish
Résumé
ABC transporters have been suggested to be involved in insecticide detoxification in different insect species mainly based on the indirect observation of transcriptional upregulation of ABC gene expression in response to insecticide exposure. Previous studies performed by us and others in the red flour beetle, Tribolium castaneum, have analyzed the function of TcABCA-C and TcABCG-H genes using RNA interference (RNAi) and demonstrated that specific TcABCA and TcABCC genes are involved in the elimination of the pyrethroid tefluthrin and the benzoylurea diflubenzuron, because gene silencing increased the beetle's susceptibility to the insecticides. In this study, we focused on the potential functions of TcABCA-C genes in detoxification of the pyrethroid cyfluthrin (CF), the organophosphate malathion (MAL) and the diacylhdyazine tebufenozide (TBF). Analysis of transcript levels of selected TcABCA-C genes in response to treatment with these three chemically unrelated insecticides revealed that some genes were particularly upregulated after insecticide treatment. In addition, the ABC inhibitor verapamil synergized significantly the toxicity of MAL but only negligibly CF and TBF toxicities. Finally, silencing of two TcABCC genes by RNAi revealed a significant increase in susceptibility to MAL. In contrast, we did not observe a significant increase in insecticide-induced mortalities when knocking down TcABC genes in larvae treated with CF or TBF, although they were upregulated in response to insecticide treatment. Our results suggest that two pleiotropic ABCC transporters expressed in metabolic and excretory tissues contribute to the elimination of MAL.
Identifiants
pubmed: 34730283
doi: 10.1111/1744-7917.12981
doi:
Substances chimiques
Insecticides
0
Pyrethrins
0
Malathion
U5N7SU872W
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1096-1104Informations de copyright
© 2021 The Authors. Insect Science published by John Wiley & Sons Australia, Ltd on behalf of Institute of Zoology, Chinese Academy of Sciences.
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