Baseline MMP expression in periapical granuloma and its relationship with periapical wound healing after surgical endodontic treatment.
MMP expression
Periapical granuloma
Periapical wound healing
Surgical endodontic treatment
Journal
BMC oral health
ISSN: 1472-6831
Titre abrégé: BMC Oral Health
Pays: England
ID NLM: 101088684
Informations de publication
Date de publication:
03 11 2021
03 11 2021
Historique:
received:
20
09
2021
accepted:
12
10
2021
entrez:
4
11
2021
pubmed:
5
11
2021
medline:
15
12
2021
Statut:
epublish
Résumé
Matrix metalloproteinases (MMPs) catalyzes the degradation of the extracellular matrix components and have a major role in many physiological processes including wound healing. In the current study, we examined the correlation of baseline MMPs 1, 2, 7, and 9 expressions with periapical wound healing after surgical endodontic treatment. 27 patients aged between 15 and 57 years presenting with chronic apical periodontitis or chronic apical abscess of an anterior tooth with previously attempted or failed root canal treatment were included in this study. During surgical endodontic treatment, tissue from the periapical lesion sample was collected and used for gross histopathological analysis as well as mRNA expression analysis of MMPs 1, 2, 7, and 9. Patients were recalled for follow-up after 6 months to evaluate the healing status both clinically and radiographically and healing was correlated with baseline MMP expression. Out of 27 patients, healing was observed in 15 patients at the end of 6 months, and in 21 patients after 12 months.. Six patients showed no healing even after 12 months. Analysis of baseline MMP 1, 2, 7, and 9 expression levels with healing status showed the mean relative expression of MMP2 and MMP9 to be considerably increased in the non-healing group as compared to the healing group. Overexpression of MMP2 and MMP9 may be considered as a potential prognostic biomarker for periapical wound healing after surgical endodontic treatment. However, further studies are desirable to establish its precise relationship with periapical wound healing.
Sections du résumé
BACKGROUND
Matrix metalloproteinases (MMPs) catalyzes the degradation of the extracellular matrix components and have a major role in many physiological processes including wound healing. In the current study, we examined the correlation of baseline MMPs 1, 2, 7, and 9 expressions with periapical wound healing after surgical endodontic treatment.
METHODS
27 patients aged between 15 and 57 years presenting with chronic apical periodontitis or chronic apical abscess of an anterior tooth with previously attempted or failed root canal treatment were included in this study. During surgical endodontic treatment, tissue from the periapical lesion sample was collected and used for gross histopathological analysis as well as mRNA expression analysis of MMPs 1, 2, 7, and 9. Patients were recalled for follow-up after 6 months to evaluate the healing status both clinically and radiographically and healing was correlated with baseline MMP expression.
RESULTS
Out of 27 patients, healing was observed in 15 patients at the end of 6 months, and in 21 patients after 12 months.. Six patients showed no healing even after 12 months. Analysis of baseline MMP 1, 2, 7, and 9 expression levels with healing status showed the mean relative expression of MMP2 and MMP9 to be considerably increased in the non-healing group as compared to the healing group.
CONCLUSION
Overexpression of MMP2 and MMP9 may be considered as a potential prognostic biomarker for periapical wound healing after surgical endodontic treatment. However, further studies are desirable to establish its precise relationship with periapical wound healing.
Identifiants
pubmed: 34732191
doi: 10.1186/s12903-021-01904-6
pii: 10.1186/s12903-021-01904-6
pmc: PMC8565031
doi:
Substances chimiques
Matrix Metalloproteinases
EC 3.4.24.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
562Informations de copyright
© 2021. The Author(s).
Références
J Endod. 2016 Jul;42(7):1082-8
pubmed: 27188765
Top Companion Anim Med. 2008 May;23(2):59-71
pubmed: 18482706
Front Biosci. 2007 Jan 01;12:1475-87
pubmed: 17127395
Semin Cell Dev Biol. 2017 Jan;61:3-11
pubmed: 27521521
J Endod. 2020 Jan;46(1):3-11.e1
pubmed: 31843126
Adv Wound Care (New Rochelle). 2015 Apr 1;4(4):225-234
pubmed: 25945285
J Endod. 2013 Apr;39(4):444-8
pubmed: 23522533
Chem Biol Interact. 2009 Aug 14;180(3):344-52
pubmed: 19426720
Vojnosanit Pregl. 2008 Dec;65(12):882-6
pubmed: 19160981
J Int Soc Prev Community Dent. 2017 Jan-Feb;7(1):64-68
pubmed: 28316952
J Inflamm (Lond). 2012 Mar 21;9(1):8
pubmed: 22436166
J Endod. 2016 Apr;42(4):533-7
pubmed: 26898567
J Oral Biol Craniofac Res. 2015 Sep-Dec;5(3):212-8
pubmed: 26605147
J Endod. 2011 Mar;37(3):316-20
pubmed: 21329814
J Endod. 2013 Sep;39(9):1141-6
pubmed: 23953287
Biochim Biophys Acta Mol Cell Res. 2017 Nov;1864(11 Pt B):2220-2227
pubmed: 28797647
Niger J Clin Pract. 2011 Jul-Sep;14(3):293-6
pubmed: 22037071
J Surg Res. 2010 Apr;159(2):633-9
pubmed: 20056248
J Endod. 2009 Sep;35(9):1234-42
pubmed: 19720222
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009 Jan;107(1):127-32
pubmed: 18926740
J Surg Res. 2011 Jun 15;168(2):315-24
pubmed: 20655064
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008 Jun;105(6):801-6
pubmed: 18439854
Int Endod J. 2004 Jun;37(6):408-16
pubmed: 15186249
J Conserv Dent. 2014 Mar;17(2):164-8
pubmed: 24778515
Int J Clin Exp Pathol. 2018 May 01;11(5):2530-2536
pubmed: 31938366
J Oral Pathol Med. 2016 Mar;45(3):224-30
pubmed: 26293377
J Endod. 2012 Feb;38(2):185-90
pubmed: 22244633
Prog Mol Biol Transl Sci. 2017;148:355-420
pubmed: 28662828