Adhesion GPCR Latrophilin 3 regulates synaptic function of cone photoreceptors in a trans-synaptic manner.

Alternative Splicing Animals Calcium Channels / metabolism Mice Mice, Knockout Protein Isoforms / metabolism Proteome Receptors, G-Protein-Coupled / genetics Receptors, Peptide / genetics Retinal Cone Photoreceptor Cells / metabolism Synapses / metabolism
adhesion GPCR horizontal cells retina synapses vision

Journal

Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876

Informations de publication

Date de publication:
09 11 2021
Historique:
accepted: 23 09 2021
entrez: 4 11 2021
pubmed: 5 11 2021
medline: 15 12 2021
Statut: ppublish

Résumé

Cone photoreceptors mediate daylight vision in vertebrates. Changes in neurotransmitter release at cone synapses encode visual information and is subject to precise control by negative feedback from enigmatic horizontal cells. However, the mechanisms that orchestrate this modulation are poorly understood due to a virtually unknown landscape of molecular players. Here, we report a molecular player operating selectively at cone synapses that modulates effects of horizontal cells on synaptic release. Using an unbiased proteomic screen, we identified an adhesion GPCR Latrophilin3 (LPHN3) in horizontal cell dendrites that engages in transsynaptic control of cones. We detected and characterized a prominent splice isoform of LPHN3 that excludes a element with inhibitory influence on transsynaptic interactions. A gain-of-function mouse model specifically routing LPHN3 splicing to this isoform but not knockout of LPHN3 diminished Ca

Identifiants

DOI: 10.1073/pnas.2106694118 PMID: 34732574 PMC: PMC8609308
pubmed: 34732574
pii: 2106694118
doi: 10.1073/pnas.2106694118
pmc: PMC8609308
pii:
doi:

Substances chimiques

Calcium Channels 0
LPHN3 protein, mouse 0
Protein Isoforms 0
Proteome 0
Receptors, G-Protein-Coupled 0
Receptors, Peptide 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NEI NIH HHS
ID : R01 EY028033
Pays : United States
Organisme : NEI NIH HHS
ID : P30 EY005722
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY024694
Pays : United States
Organisme : NEI NIH HHS
ID : R29 EY010542
Pays : United States
Organisme : NEI NIH HHS
ID : K99 EY030554
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY018139
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY031445
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY010542
Pays : United States
Organisme : NEI NIH HHS
ID : F32 EY026344
Pays : United States

Déclaration de conflit d'intérêts

The authors declare no competing interest.

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Auteurs

Yuchen Wang (Y)

Department of Neuroscience, The Scripps Research Institute, Jupiter, FL 33458.
ORCID: 0000-0001-5246-7305

Yan Cao (Y)

Department of Neuroscience, The Scripps Research Institute, Jupiter, FL 33458.
ORCID: 0000-0003-4902-2297

Cassandra L Hays (CL)

Truhlsen Eye Institute, Durham Research Center I, University of Nebraska Medical Center, Omaha, NE 68198-5840.
ORCID: 0000-0003-4481-3495

Thibaut Laboute (T)

Department of Neuroscience, The Scripps Research Institute, Jupiter, FL 33458.
ORCID: 0000-0003-0870-1891

Thomas A Ray (TA)

Department of Neurobiology and Ophthalmology, and Cell Biology, Duke University School of Medicine, Durham, NC 27710.

Debbie Guerrero-Given (D)

Electron Microscopy Core Facility, Max Planck Florida Institute, Jupiter, FL 33458.

Abhimanyu S Ahuja (AS)

Department of Neuroscience, The Scripps Research Institute, Jupiter, FL 33458.
ORCID: 0000-0003-4333-9189

Dipak Patil (D)

Department of Neuroscience, The Scripps Research Institute, Jupiter, FL 33458.

Olga Rivero (O)

Division of Molecular Psychiatry, Center of Mental Health, University of Würzburg 97080 Würzburg, Germany.

Naomi Kamasawa (N)

Electron Microscopy Core Facility, Max Planck Florida Institute, Jupiter, FL 33458.
ORCID: 0000-0002-8926-5309

Jeremy N Kay (JN)

Department of Neurobiology and Ophthalmology, and Cell Biology, Duke University School of Medicine, Durham, NC 27710.
ORCID: 0000-0001-6145-1604

Wallace B Thoreson (WB)

Truhlsen Eye Institute, Durham Research Center I, University of Nebraska Medical Center, Omaha, NE 68198-5840.
ORCID: 0000-0001-7104-042X

Kirill A Martemyanov (KA)

Department of Neuroscience, The Scripps Research Institute, Jupiter, FL 33458; kirill@scripps.edu.
ORCID: 0000-0002-9925-7599

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Maturation post-transcriptionnelle des ARN › Épissage des ARN › Épissage alternatif › Adhesion GPCR Latrophilin 3 regulates synaptic...
questionsmedicales.fr › Eucaryotes › Animaux › Adhesion GPCR Latrophilin 3 regulates synaptic...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Canaux ioniques › Canaux calciques › Adhesion GPCR Latrophilin 3 regulates synaptic...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Adhesion GPCR Latrophilin 3 regulates synaptic...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Souris transgéniques › Souris knockout › Adhesion GPCR Latrophilin 3 regulates synaptic...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Isoformes de protéines › Adhesion GPCR Latrophilin 3 regulates synaptic...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéome › Adhesion GPCR Latrophilin 3 regulates synaptic...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Récepteurs couplés aux protéines G › Adhesion GPCR Latrophilin 3 regulates synaptic...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Récepteurs peptidiques › Adhesion GPCR Latrophilin 3 regulates synaptic...
questionsmedicales.fr › Cellules › Neurones › Neurones afférents › Cellules réceptrices sensorielles › Cellules photoréceptrices › Cellules photoréceptrices de vertébré › Cellules photoréceptrices en cône de la rétine › Adhesion GPCR Latrophilin 3 regulates synaptic...
questionsmedicales.fr › Cellules › Structures cellulaires › Membrane cellulaire › Structures de la membrane cellulaire › Jonctions intercellulaires › Synapses › Adhesion GPCR Latrophilin 3 regulates synaptic...