The relationship of smoking to cg05575921 methylation in blood and saliva DNA samples from several studies.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
03 11 2021
03 11 2021
Historique:
received:
07
07
2021
accepted:
18
10
2021
entrez:
4
11
2021
pubmed:
5
11
2021
medline:
28
1
2022
Statut:
epublish
Résumé
Numerous studies have shown that cg05575921 methylation decreases in response to smoking. However, secondary to methodological issues, the magnitude and dose dependency of that response is as of yet unclear. This lack of certainty is a barrier to the use of DNA methylation clinically to assess and monitor smoking status. To better define this relationship, we conducted a joint analysis of methylation sensitive PCR digital (MSdPCR) assessments of cg05575921 methylation in whole blood and/or saliva DNA to smoking using samples from 421 smokers and 423 biochemically confirmed non-smokers from 4 previously published studies. We found that cg05575921 methylation manifested a curvilinear dose dependent decrease in response to increasing cigarette consumption. In whole blood DNA, the Receiver Operating Characteristic (ROC) Area Under the Curve (AUC) of cg05575921 methylation for predicting daily smoking status was 0.98. In saliva DNA, the gross AUC was 0.91 with correction for cellular heterogeneity improving the AUC to 0.94. Methylation status was significantly associated with the Fagerstrom Test for Nicotine Dependence score, but with significant sampling heterogeneity. We conclude that MSdPCR assessments of cg05575921 methylation are a potentially powerful, clinically implementable tool for the assessment and management of smoking.
Identifiants
pubmed: 34732805
doi: 10.1038/s41598-021-01088-7
pii: 10.1038/s41598-021-01088-7
pmc: PMC8566492
doi:
Substances chimiques
AHRR protein, human
0
Basic Helix-Loop-Helix Transcription Factors
0
Repressor Proteins
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
21627Subventions
Organisme : NCI NIH HHS
ID : R44 CA213507
Pays : United States
Organisme : NIAAA NIH HHS
ID : R44 AA022041
Pays : United States
Informations de copyright
© 2021. The Author(s).
Références
Am J Med Genet B Neuropsychiatr Genet. 2020 Jan;183(1):51-60
pubmed: 31456352
Ther Drug Monit. 2009 Feb;31(1):14-30
pubmed: 19125149
Transl Psychiatry. 2019 Feb 15;9(1):92
pubmed: 30770782
PLoS One. 2011;6(12):e28864
pubmed: 22194931
BMC Genomics. 2014 Feb 22;15:151
pubmed: 24559495
J Insur Med. 2019;48(1):90-102
pubmed: 31609642
BMC Med Res Methodol. 2021 Jan 7;21(1):9
pubmed: 33413132
Genes (Basel). 2019 Jan 15;10(1):
pubmed: 30650672
Epidemiology. 2010 Jan;21(1):128-38
pubmed: 20010215
Front Psychiatry. 2015 Sep 22;6:132
pubmed: 26441693
Thorax. 2017 Jul;72(7):646-653
pubmed: 28100713
BMC Genomics. 2009 Mar 19;10:116
pubmed: 19298667
Front Psychiatry. 2016 Apr 06;7:55
pubmed: 27092088
PLoS One. 2013 May 17;8(5):e63812
pubmed: 23691101
Epigenomics. 2019 Oct;11(13):1469-1486
pubmed: 31466478
Clin Epigenetics. 2020 Jun 24;12(1):92
pubmed: 32580755
Environ Int. 2016 Dec;97:163-170
pubmed: 27613001
Brief Bioinform. 2014 Nov;15(6):929-41
pubmed: 23990268
EBioMedicine. 2018 Nov;37:214-220
pubmed: 30389506
Occup Med (Lond). 2018 May 23;68(4):255-261
pubmed: 29579260
Genes (Basel). 2020 Nov 27;11(12):
pubmed: 33260961
Mol Cell. 2013 Jan 24;49(2):359-367
pubmed: 23177740
Front Genet. 2018 Apr 24;9:137
pubmed: 29740475
J Insur Med. 2019;48(1):79-89
pubmed: 31618096
Epigenetics. 2014 Sep;9(9):1212-9
pubmed: 25147915
J Child Adolesc Psychopharmacol. 2019 Aug;29(7):535-544
pubmed: 31180231
Nat Biotechnol. 2016 Jul;34(7):726-37
pubmed: 27347756
Front Psychol. 2015 Jun 02;6:656
pubmed: 26082730
Toxicol Sci. 2015 May;145(1):5-15
pubmed: 25911656
Arch Toxicol. 2019 Oct;93(10):2715-2740
pubmed: 31555878
Br J Addict. 1991 Sep;86(9):1119-27
pubmed: 1932883
Cochrane Database Syst Rev. 2019 Apr 18;4:CD013308
pubmed: 30997928
Proc Natl Acad Sci U S A. 2005 Jul 26;102(30):10604-9
pubmed: 16009939
Hum Mol Genet. 2015 Apr 15;24(8):2349-59
pubmed: 25556184
Clin Epigenetics. 2020 Jul 31;12(1):117
pubmed: 32736658
BMC Genomics. 2017 Oct 18;18(1):805
pubmed: 29047347
Aging (Albany NY). 2019 Jan 21;11(2):303-327
pubmed: 30669119
J Pharmacol Exp Ther. 1987 Feb;240(2):554-64
pubmed: 3806411
Environ Pollut. 2013 Feb;173:150-6
pubmed: 23202645
Am J Med Genet B Neuropsychiatr Genet. 2015 Jan;168B(1):36-44
pubmed: 25355443
J Biomed Inform. 2019 Jul;95:103208
pubmed: 31078660
Clin Epigenetics. 2021 Apr 29;13(1):98
pubmed: 33926513
Epigenetics. 2021 Feb 25;:1-13
pubmed: 33588690
Genes (Basel). 2015 Oct 14;6(4):991-1022
pubmed: 26473933
Int J Cancer. 2012 Dec 15;131(12):2724-32
pubmed: 22945513
Environ Res. 2016 Apr;146:395-403
pubmed: 26826776
PLoS One. 2011;6(6):e14821
pubmed: 21731603
J Stud Alcohol. 1994 Mar;55(2):149-58
pubmed: 8189735
Am J Med Genet B Neuropsychiatr Genet. 2017 Sep;174(6):641-650
pubmed: 28816414
Anal Chem. 2011 Nov 15;83(22):8604-10
pubmed: 22035192