Zfhx4 regulates endochondral ossification as the transcriptional platform of Osterix in mice.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
03 11 2021
03 11 2021
Historique:
received:
30
01
2020
accepted:
18
10
2021
entrez:
4
11
2021
pubmed:
5
11
2021
medline:
21
12
2021
Statut:
epublish
Résumé
Endochondral ossification is regulated by transcription factors that include SRY-box transcription factor 9, runt-related protein 2 (Runx2), and Osterix. However, the sequential and harmonious regulation of the multiple steps of endochondral ossification is unclear. This study identified zinc finger homeodomain 4 (Zfhx4) as a crucial transcriptional partner of Osterix. We found that Zfhx4 was highly expressed in cartilage and that Zfhx4 deficient mice had reduced expression of matrix metallopeptidase 13 and inhibited calcification of cartilage matrices. These phenotypes were very similar to impaired chondrogenesis in Osterix deficient mice. Coimmunoprecipitation and immunofluorescence indicated a physical interaction between Zfhx4 and Osterix. Notably, Zfhx4 and Osterix double mutant mice showed more severe phenotype than Zfhx4 deficient mice. Additionally, Zfhx4 interacted with Runx2 that functions upstream of Osterix. Our findings suggest that Zfhx4 coordinates the transcriptional network of Osterix and, consequently, endochondral ossification.
Identifiants
pubmed: 34732852
doi: 10.1038/s42003-021-02793-9
pii: 10.1038/s42003-021-02793-9
pmc: PMC8566502
doi:
Substances chimiques
Homeodomain Proteins
0
Sp7 Transcription Factor
0
Sp7 protein, mouse
0
Zfhx4 protein, mouse
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1258Subventions
Organisme : Japan Society for the Promotion of Science London (JSPS London)
ID : 21H04841
Organisme : Japan Society for the Promotion of Science London (JSPS London)
ID : A16H063930
Organisme : Japan Society for the Promotion of Science London (JSPS London)
ID : T20K204750
Informations de copyright
© 2021. The Author(s).
Références
Endocrinology. 2005 Mar;146(3):1012-7
pubmed: 15604211
Dev Cell. 2001 Aug;1(2):277-90
pubmed: 11702786
J Cell Sci. 2010 Nov 1;123(Pt 21):3780-8
pubmed: 20940257
Development. 2004 Jul;131(13):3207-16
pubmed: 15175245
Nat Genet. 2002 Dec;32(4):645-9
pubmed: 12434155
J Histochem Cytochem. 2004 Jan;52(1):131-9
pubmed: 14688224
Genetics. 2014 Nov;198(3):1117-26
pubmed: 25217052
Nat Commun. 2011;2:251
pubmed: 21427722
Genesis. 2012 Nov;50(11):819-27
pubmed: 22644989
Cell. 1994 Dec 16;79(6):1111-20
pubmed: 8001137
Anal Biochem. 1993 Oct;214(1):70-6
pubmed: 8250257
Cell Rep. 2014 Jan 30;6(2):313-24
pubmed: 24440720
Nat Genet. 2002 Dec;32(4):639-44
pubmed: 12434156
Am J Hum Genet. 2002 Mar;70(3):612-24
pubmed: 11781871
Mech Dev. 2001 Aug;106(1-2):97-106
pubmed: 11472838
Proc Natl Acad Sci U S A. 2011 Mar 22;108(12):4888-91
pubmed: 21383175
Mol Biol Cell. 2011 Apr 15;22(8):1300-11
pubmed: 21346191
Nat Cell Biol. 2011 Jan;13(1):59-65
pubmed: 21170031
Lancet. 2007 Jul 14;370(9582):162-172
pubmed: 17630040
J Bone Miner Res. 2002 Jul;17(7):1297-305
pubmed: 12096844
Nat Genet. 1999 May;22(1):85-9
pubmed: 10319868
Protein Expr Purif. 2008 Jun;59(2):232-41
pubmed: 18375142
Genes Dev. 2002 Nov 1;16(21):2813-28
pubmed: 12414734
Dev Dyn. 1999 Apr;214(4):279-90
pubmed: 10213384
Development. 1996 Feb;122(2):461-72
pubmed: 8625797
Proc Natl Acad Sci U S A. 2005 Feb 15;102(7):2414-9
pubmed: 15699338
J Cell Biol. 2003 Sep 1;162(5):833-42
pubmed: 12952936
Birth Defects Res C Embryo Today. 2005 Sep;75(3):200-12
pubmed: 16187326
Science. 2003 Jul 18;301(5631):363-7
pubmed: 12869760
Nature. 2003 May 15;423(6937):332-6
pubmed: 12748651
Sci Rep. 2018 Jul 10;8(1):10375
pubmed: 29991769
Curr Opin Cell Biol. 2001 Dec;13(6):721-7
pubmed: 11698188
Nat Rev Mol Cell Biol. 2011 Dec 22;13(1):27-38
pubmed: 22189423
Nat Commun. 2015 Mar 26;6:6653
pubmed: 25808752
J Biol Chem. 1993 Jul 15;268(20):15238-45
pubmed: 8325895
Nat Genet. 1994 Apr;6(4):348-56
pubmed: 7914451
Mol Cell Biol. 1997 Apr;17(4):2336-46
pubmed: 9121483
Science. 2004 Dec 24;306(5705):2255-7
pubmed: 15618518
Nat Genet. 2009 Mar;41(3):359-64
pubmed: 19234473
Nat Commun. 2013;4:2850
pubmed: 24276541
J Clin Invest. 2008 Sep;118(9):3098-108
pubmed: 18677406
Gene. 1996 Feb 12;168(2):227-31
pubmed: 8654949
J Cell Sci. 2012 Dec 15;125(Pt 24):6071-83
pubmed: 23038774
Development. 2002 Sep;129(17):4135-46
pubmed: 12163415
J Comp Neurol. 2005 Jan 31;482(1):33-49
pubmed: 15612017
Gene. 2006 May 10;372:62-70
pubmed: 16574347
Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9360-5
pubmed: 12878728
Nature. 1995 Jun 29;375(6534):791-5
pubmed: 7596412
J Clin Invest. 2004 Jun;113(12):1692-700
pubmed: 15199404
J Cell Biol. 2004 Mar 1;164(5):747-58
pubmed: 14993235
J Biol Chem. 2012 Sep 28;287(40):33179-90
pubmed: 22869368
Cell. 1997 May 30;89(5):755-64
pubmed: 9182763
EMBO J. 1998 Oct 1;17(19):5718-33
pubmed: 9755172
J Bone Miner Metab. 2011 Jul;29(4):390-5
pubmed: 21594584
Am J Hum Genet. 2011 Aug 12;89(2):295-301
pubmed: 21802062
J Biol Chem. 2000 Apr 14;275(15):10738-44
pubmed: 10753864
J Biol Chem. 2005 Oct 21;280(42):35203-8
pubmed: 16109717
World J Radiol. 2014 Oct 28;6(10):808-25
pubmed: 25349664
Eur J Biochem. 1993 Apr 1;213(1):99-111
pubmed: 8477738
Dev Biol. 2009 Apr 15;328(2):200-9
pubmed: 19389375
Mol Cell Biol. 2005 Mar;25(5):1971-9
pubmed: 15713650
Development. 2013 Dec;140(23):4709-18
pubmed: 24173808
Nature. 1994 Dec 8;372(6506):525-30
pubmed: 7990924
Mol Cell Biol. 1999 Jun;19(6):4431-42
pubmed: 10330183
Genes Dev. 2004 Apr 15;18(8):952-63
pubmed: 15107406
Genes Dev. 1998 Sep 1;12(17):2735-47
pubmed: 9732271