Dishevelled-1 DIX and PDZ domain lysine residues regulate oncogenic Wnt signaling.

chromatin immunoprecipitation (ChIP) dishevelled (DVL) gene expression lysine residue post-translational modification

Journal

Oncotarget
ISSN: 1949-2553
Titre abrégé: Oncotarget
Pays: United States
ID NLM: 101532965

Informations de publication

Date de publication:
26 Oct 2021
Historique:
received: 03 08 2021
accepted: 24 09 2021
entrez: 4 11 2021
pubmed: 5 11 2021
medline: 5 11 2021
Statut: epublish

Résumé

DVL proteins are central mediators of the Wnt pathway and relay complex input signals into different branches of the Wnt signaling network. However, molecular mechanism(s) that regulate DVL-mediated relay of Wnt signals still remains unclear. Here, for the first time, we elucidate the functional significance of three DVL-1 lysines (K/Lys) which are subject to post-translational acetylation. We demonstrate that K34 Lys residue in the DIX domain regulates subcellular localization of β-catenin, thereby influencing downstream Wnt target gene expression. Additionally, we show that K69 (DIX domain) and K285 (PDZ domain) regulate binding of DVL-1 to Wnt target gene promoters and modulate expression of Wnt target genes including

Identifiants

pubmed: 34733415
doi: 10.18632/oncotarget.28089
pii: 28089
pmc: PMC8555683
doi:

Types de publication

Journal Article

Langues

eng

Pagination

2234-2251

Subventions

Organisme : NCI NIH HHS
ID : R01 CA155223
Pays : United States

Informations de copyright

Copyright: © 2021 Sharma et al.

Déclaration de conflit d'intérêts

CONFLICTS OF INTEREST Authors have no conflicts of interest to declare.

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Auteurs

Monica Sharma (M)

Immunology and Molecular Microbiology, Texas Tech University Health Sciences Center, Lubbock, TX, USA.

Isabel Castro-Piedras (I)

Immunology and Molecular Microbiology, Texas Tech University Health Sciences Center, Lubbock, TX, USA.

Fahmida Rasha (F)

Immunology and Molecular Microbiology, Texas Tech University Health Sciences Center, Lubbock, TX, USA.

Sabarish Ramachandran (S)

Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX, USA.

Souad R Sennoune (SR)

Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX, USA.

Kathryn Furr (K)

Immunology and Molecular Microbiology, Texas Tech University Health Sciences Center, Lubbock, TX, USA.

Sharilyn Almodovar (S)

Immunology and Molecular Microbiology, Texas Tech University Health Sciences Center, Lubbock, TX, USA.

Vadivel Ganapathy (V)

Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX, USA.

Matthew B Grisham (MB)

Immunology and Molecular Microbiology, Texas Tech University Health Sciences Center, Lubbock, TX, USA.

Rakhshanda Layeequr Rahman (RL)

Department of Surgery, Texas Tech University Health Sciences Center, Lubbock, TX, USA.

Kevin Pruitt (K)

Immunology and Molecular Microbiology, Texas Tech University Health Sciences Center, Lubbock, TX, USA.

Classifications MeSH