Photocleavable Fluorescent Membrane Tension Probes: Fast Release with Spatiotemporal Control in Inner Leaflets of Plasma Membrane, Nuclear Envelope, and Secretory Pathway.
fluorescent probes
mechanosensitivity
membrane asymmetry
membrane tension
photocleavage
Journal
Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543
Informations de publication
Date de publication:
03 01 2022
03 01 2022
Historique:
revised:
25
10
2021
received:
28
09
2021
pubmed:
5
11
2021
medline:
18
1
2022
entrez:
4
11
2021
Statut:
ppublish
Résumé
Mechanosensitive flipper probes are attracting interest as fluorescent reporters of membrane order and tension in biological systems. We introduce PhotoFlippers, which contain a photocleavable linker and an ultralong tether between mechanophore and various targeting motifs. Upon irradiation, the original probe is released and labels the most ordered membrane that is accessible by intermembrane transfer. Spatiotemporal control from photocleavable flippers is essential to access open, dynamic or elusive membrane motifs without chemical or physical interference. For instance, fast release with light is shown to place the original small-molecule probes into the innermost leaflet of the nuclear envelope to image changes in membrane tension, at specific points in time of membrane trafficking along the secretory pathway, or in the inner leaflet of the plasma membrane to explore membrane asymmetry. These results identify PhotoFlippers as useful chemistry tools to enable research in biology.
Identifiants
pubmed: 34734671
doi: 10.1002/anie.202113163
pmc: PMC9299180
doi:
Substances chimiques
Fluorescent Dyes
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202113163Subventions
Organisme : Swiss NSF
ID : 175486 and 204175
Organisme : Swiss NSF
ID : 189246
Organisme : National Centre of Competence in Research (NCCR)
ID : Chemical Biology / 51NF40-185898
Organisme : National Centre of Competence in Research (NCCR)
ID : Molecular Systems Engineering / 51NF40-18289
Organisme : Université de Genève
ID : DIP
Organisme : Alfred Werner Foundation
ID : MS Fellowship
Informations de copyright
© 2021 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.
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