Some living eukaryotes during and after scanning electron microscopy.
Desiccation
Radiation
Scanning electron microscopy
Vacuum
Journal
Applied microscopy
ISSN: 2287-4445
Titre abrégé: Appl Microsc
Pays: Korea (South)
ID NLM: 101698575
Informations de publication
Date de publication:
04 Nov 2021
04 Nov 2021
Historique:
received:
13
09
2021
accepted:
25
10
2021
entrez:
4
11
2021
pubmed:
5
11
2021
medline:
5
11
2021
Statut:
epublish
Résumé
Electron microscopy (EM) is an essential imaging method in biological sciences. Since biological specimens are exposed to radiation and vacuum conditions during EM observations, they die due to chemical bond breakage and desiccation. However, some organisms belonging to the taxa of bacteria, fungi, plants, and animals (including beetles, ticks, and tardigrades) have been reported to survive hostile scanning EM (SEM) conditions since the onset of EM. The surviving organisms were observed (i) without chemical fixation, (ii) after mounting to a precooled cold stage, (iii) using cryo-SEM, or (iv) after coating with a thin polymer layer, respectively. Combined use of these techniques may provide a better condition for preservation and live imaging of multicellular organisms for a long time beyond live-cell EM.
Identifiants
pubmed: 34735657
doi: 10.1186/s42649-021-00065-8
pii: 10.1186/s42649-021-00065-8
pmc: PMC8569123
doi:
Types de publication
Journal Article
Review
Langues
eng
Pagination
16Informations de copyright
© 2021. The Author(s).
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