Z-form extracellular DNA is a structural component of the bacterial biofilm matrix.
Animals
Antibody Specificity
Bacteria
/ genetics
Bacterial Proteins
/ metabolism
Biofilms
Cell Line
Chinchilla
DNA, Bacterial
/ chemistry
DNA, Cruciform
Deoxyribonucleases
/ metabolism
Extracellular Matrix
/ metabolism
Extracellular Space
/ chemistry
Extracellular Traps
/ metabolism
Humans
Tetradecanoylphorbol Acetate
/ pharmacology
DNABII proteins
DNase resistance
Z-DNA
biofilm matrix
extracellular DNA
Journal
Cell
ISSN: 1097-4172
Titre abrégé: Cell
Pays: United States
ID NLM: 0413066
Informations de publication
Date de publication:
11 11 2021
11 11 2021
Historique:
received:
22
01
2021
revised:
03
08
2021
accepted:
12
10
2021
pubmed:
5
11
2021
medline:
7
1
2022
entrez:
4
11
2021
Statut:
ppublish
Résumé
Biofilms are community architectures adopted by bacteria inclusive of a self-formed extracellular matrix that protects resident bacteria from diverse environmental stresses and, in many species, incorporates extracellular DNA (eDNA) and DNABII proteins for structural integrity throughout biofilm development. Here, we present evidence that this eDNA-based architecture relies on the rare Z-form. Z-form DNA accumulates as biofilms mature and, through stabilization by the DNABII proteins, confers structural integrity to the biofilm matrix. Indeed, substances known to drive B-DNA into Z-DNA promoted biofilm formation whereas those that drive Z-DNA into B-DNA disrupted extant biofilms. Importantly, we demonstrated that the universal bacterial DNABII family of proteins stabilizes both bacterial- and host-eDNA in the Z-form in situ. A model is proposed that incorporates the role of Z-DNA in biofilm pathogenesis, innate immune response, and immune evasion.
Identifiants
pubmed: 34735796
pii: S0092-8674(21)01220-4
doi: 10.1016/j.cell.2021.10.010
pmc: PMC8595767
mid: NIHMS1748889
pii:
doi:
Substances chimiques
Bacterial Proteins
0
DNA, Bacterial
0
DNA, Cruciform
0
Deoxyribonucleases
EC 3.1.-
Tetradecanoylphorbol Acetate
NI40JAQ945
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5740-5758.e17Subventions
Organisme : NIAID NIH HHS
ID : R01 AI155501
Pays : United States
Organisme : NIDCD NIH HHS
ID : R01 DC011818
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM124436
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests S.D.G. and L.O.B. are founders, shareholders, and members of the scientific advisory board of Clarametyx Biosciences, Inc.
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