Immobilization of Antibodies by Genetic Fusion to a Fungal Self-Assembling Adhesive Protein.
ScFv
algal toxins
biosensors
electrochemical detection
hydrophobin
optical detection
Journal
Frontiers in molecular biosciences
ISSN: 2296-889X
Titre abrégé: Front Mol Biosci
Pays: Switzerland
ID NLM: 101653173
Informations de publication
Date de publication:
2021
2021
Historique:
received:
15
06
2021
accepted:
14
09
2021
entrez:
5
11
2021
pubmed:
6
11
2021
medline:
6
11
2021
Statut:
epublish
Résumé
Although antibody immobilization on solid surfaces is extensively used in several applications, including immunoassays, biosensors, and affinity chromatography, some issues are still challenging. Self-assembling protein layers can be used to coat easily different surfaces by direct deposition. A specific biofunctional layer can be formed using genetic engineering techniques to express fused proteins acting as self-immobilizing antibodies. In this study, fusion proteins combining the self-assembling adhesive properties of a fungal hydrophobin and the functionality of the single chain fragment variables (ScFvs) of two antibodies were produced. The chosen ScFvs are able to recognize marine toxins associated with algal blooms, saxitoxin, and domoic acid, which can bioaccumulate in shellfish and herbivorous fish causing food poisoning. ScFvs fused to hydrophobin Vmh2 from
Identifiants
pubmed: 34738014
doi: 10.3389/fmolb.2021.725697
pii: 725697
pmc: PMC8561437
doi:
Types de publication
Journal Article
Langues
eng
Pagination
725697Informations de copyright
Copyright © 2021 Stanzione, Izquierdo-Bote, González García, Giardina and Piscitelli.
Déclaration de conflit d'intérêts
Authors DIB and MBGG are employed by the company Metrohm DropSens. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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