Identification of genotypic variants and its proteomic mutations of Brazilian SARS-CoV-2 isolates.
Brazil
COVID-19
/ pathology
Coronavirus 3C Proteases
/ genetics
Coronavirus Nucleocapsid Proteins
/ genetics
Coronavirus RNA-Dependent RNA Polymerase
/ genetics
Genetic Variation
/ genetics
Genome, Viral
/ genetics
Humans
Phosphoproteins
/ genetics
Protein Folding
SARS-CoV-2
/ genetics
Spike Glycoprotein, Coronavirus
/ genetics
Viral Nonstructural Proteins
/ genetics
Virulence
/ genetics
Brazilian isolates
Genotypic variants
Phylogenomic study
Protein mutations
SARS-CoV-2
Journal
Virus research
ISSN: 1872-7492
Titre abrégé: Virus Res
Pays: Netherlands
ID NLM: 8410979
Informations de publication
Date de publication:
02 01 2022
02 01 2022
Historique:
received:
06
10
2021
revised:
25
10
2021
accepted:
26
10
2021
pubmed:
7
11
2021
medline:
15
12
2021
entrez:
6
11
2021
Statut:
ppublish
Résumé
The second wave of COVID-19 caused by severe acute respiratory syndrome virus (SARS-CoV-2) is rapidly spreading over the world. Mechanisms behind the flee from current antivirals are still unclear due to the continuous occurrence of SARS-CoV-2 genetic variants. Brazil is the world's second-most COVID-19 affected country. In the present study, we identified the genomic and proteomic variants of Brazilian SARS-CoV-2 isolates. We identified 16 different genotypic variants were found among the 27 isolates. The genotypes of three isolates such as Bra/1236/2021 (G15), Bra/MASP2C844R2/2020 (G11), and Bra/RJ-DCVN5/2020 (G9) have a unique mutant in NSP4 (S184N), 2'O-Mutase (R216N), membrane protein (A2V) and Envelope protein (V5A). A mutation in RdRp of SARS-CoV-2, particularly the change of Pro-to Leu-at 323 resulted in the stabilization of the structure in BRA/CD1739-P4/2020. NSP4, NSP5 protein mutants are more virulent in genotype 15 and 16. A fast protein folding rate changes the structural stability and leads to escape for current antivirals. Thus, our findings help researchers to develop the best potent antivirals based on the new mutant of Brazilian isolates.
Identifiants
pubmed: 34740719
pii: S0168-1702(21)00325-7
doi: 10.1016/j.virusres.2021.198618
pmc: PMC8563081
pii:
doi:
Substances chimiques
Coronavirus Nucleocapsid Proteins
0
NSP4 protein, SARS-CoV-2
0
Phosphoproteins
0
Spike Glycoprotein, Coronavirus
0
Viral Nonstructural Proteins
0
nucleocapsid phosphoprotein, SARS-CoV-2
0
spike protein, SARS-CoV-2
0
Coronavirus RNA-Dependent RNA Polymerase
EC 2.7.7.48
NSP12 protein, SARS-CoV-2
EC 2.7.7.48
3C-like proteinase, SARS-CoV-2
EC 3.4.22.-
Coronavirus 3C Proteases
EC 3.4.22.28
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
198618Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.
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